Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 and 25 mM, 25 and 50% inhibition of thiolysis | Rhizobium sp. | |
CoA | substrate inhibition above 0.05 mM, 50% inhibition of synthesis at 0.06 mM in bacteroids | Rhizobium sp. | |
Mg2+ | 2 and 5 mM, 20 and 40% inhibition of synthesis, respectively | Rhizobium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
acetoacetyl-CoA | thiolysis | Rhizobium sp. | |
0.056 | - |
CoA | thiolysis | Rhizobium sp. | |
0.38 | - |
acetyl-CoA | enzyme from bacteroids | Rhizobium sp. | |
1.06 | - |
acetyl-CoA | synthesis | Rhizobium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can replace Mg2+, 5 mM, 90% of activity with Mg2+, inhibition above 10 mM | Rhizobium sp. | |
Mg2+ | maximal activity at 5-10 mM, inhibition above | Rhizobium sp. | |
Mn2+ | can replace Mg2+, 5 mM, 90% of activity with Mg2+, inhibition above | Rhizobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE | Rhizobium sp. |
180000 | - |
gel filtration | Rhizobium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | Rhizobium sp. | - |
acetyl-CoA + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Econo-Q, Superose 6, Mono Q, HA-Ultrogel, Mono Q | Rhizobium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bacteroid | - |
Rhizobium sp. | - |
free-living state | - |
Rhizobium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
163 | - |
- |
Rhizobium sp. |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM 1 mM, EDTA, 15 d, loss of more than 50% activity | Rhizobium sp. |
-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 1 mM, EDTA, 15 d, loss of 20% activity | Rhizobium sp. |
-20°C, 20 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 1 mM, EDTA, 20% glycerol, 60 d, loss of less than 20% activity | Rhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + acetoacetyl-CoA | - |
Rhizobium sp. | acetyl-CoA + acetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | - |
Rhizobium sp. | acetyl-CoA + acetyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, SDS-PAGE | Rhizobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
thiolysis, more than 90% of maximal activity between pH 7.5 and pH 8.3 | Rhizobium sp. |
8.1 | - |
synthesis, more than 90% of maximal activity between pH 7.5 and pH 8.5 | Rhizobium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
CoA | enzyme from bacteroids | Rhizobium sp. | |
0.011 | - |
CoA | - |
Rhizobium sp. |