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Literature summary for 2.3.1.74 extracted from

  • Aiswarya, G.; Mallika, V.; Mur, L.A.; Soniya, E.V.
    Ectopic expression and functional characterization of type III polyketide synthase mutants from Emblica officinalis Gaertn (2016), Plant Cell Rep., 35, 2077-2090 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Phyllanthus emblica

Protein Variants

Protein Variants Comment Organism
F215S functionally active mutant that prefers N-methylanthraniloyl-CoA over p-coumaroyl-CoA. Analyses of tobacco leaves transiently expressing mutant genes show high levels of naringenin, acridones and quinolone derivatives compared to wild-type enzyme Phyllanthus emblica
F265V functionally active mutant that prefers N-methylanthraniloyl-CoA over p-coumaroyl-CoA. Analyses of tobacco leaves transiently expressing mutant genes show high levels of naringenin, acridones and quinolone derivatives compared to wild-type enzyme Phyllanthus emblica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Phyllanthus emblica 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42560
-
calculated from sequence Phyllanthus emblica

Organism

Organism UniProt Comment Textmining
Phyllanthus emblica W8QI55
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phyllanthus emblica

pI Value

Organism Comment pI Value Maximum pI Value
Phyllanthus emblica calculated from sequence
-
6.11