Application | Comment | Organism |
---|---|---|
medicine | there is increasing evidence that arylamine N-acetyltransferases could contribute to antibiotic resistance in pathogenic bacteria | Nocardia farcinica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Nocardia farcinica |
into the pET28a vector for expression in Escherichia coli BL21DE3 pLysS cells | Nocardia farcinica |
Crystallization (Comment) | Organism |
---|---|
crystal structure of NfNAT is solved at a resolution of 2.7 A. Despite low sequence identity, enzyme shares an almost identical fold with that of other prokaryotic NATs, e.g. Mycobacterium smegmatis NAT and Mycobacterium marinum NAT. The overall NfNAT structure consists of three domains of equivalent size. The first two domains, a helical bundle (amino acids 1-98) and a beta-barrel (amino acids 99-199), are disposed in such a way that three residues (Cys82, His119, and Asp136) form a catalytic triad. The third domain is linked to the second through an interdomain helix (alpha6: amino acids 200-210). As shown for other prokaryotic NAT structures, the interface formed between domains 2 and 3 forms a substantial active-site cleft | Nocardia farcinica |
the structure of NfNAT is solved at a resolution of 2.7 A | Nocardia farcinica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.414 | - |
isoniazid | - |
Nocardia farcinica | |
1.414 | - |
isoniazid | apparent KM | Nocardia farcinica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
SDS-PAGE | Nocardia farcinica |
36000 | - |
determined by SDS-PAGE and Western Blot analysis | Nocardia farcinica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia farcinica | - |
- |
- |
Nocardia farcinica | Q5YYQ3 | - |
- |
Purification (Comment) | Organism |
---|---|
using HIS-Select resin | Nocardia farcinica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.013 | - |
substrate: 2-aminofluorene | Nocardia farcinica |
0.015 | - |
sulfamethazine | Nocardia farcinica |
0.015 | - |
substrate: sulfamethazine | Nocardia farcinica |
0.023 | - |
substrate: beta-naphthylamine | Nocardia farcinica |
0.024 | - |
substrate: 4-aminobiphenyl | Nocardia farcinica |
0.02463 | - |
substrate: 5-aminosalicylate | Nocardia farcinica |
0.029 | - |
substrate: sulfamethoxazole | Nocardia farcinica |
0.029 | - |
sulfamethoxazole | Nocardia farcinica |
0.052 | - |
substrate: benzidine | Nocardia farcinica |
0.079 | - |
substrate: 4-aminoveratrole | Nocardia farcinica |
0.087 | - |
substrate: hydralazine | Nocardia farcinica |
0.111 | - |
4-aminobenzoic acid | Nocardia farcinica |
0.111 | - |
substrate: 4-aminobenzoic acid | Nocardia farcinica |
0.372 | - |
4-aminosalicylate | Nocardia farcinica |
0.372 | - |
substrate: 4-aminosalicylate | Nocardia farcinica |
2.878 | - |
isoniazid | Nocardia farcinica |
2.878 | - |
substrate: isoniazid | Nocardia farcinica |
13.03 | - |
4-aminofluorene | Nocardia farcinica |
23.73 | - |
beta-naphthylamine | Nocardia farcinica |
24.63 | - |
5-aminosalicylate | Nocardia farcinica |
24.91 | - |
2-aminobiphenyl | Nocardia farcinica |
52.33 | - |
benzidine | Nocardia farcinica |
79.39 | - |
4-aminoveratrole | Nocardia farcinica |
87.76 | - |
hydralazine | Nocardia farcinica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-aminobiphenyl | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + 2-aminofluorene | - |
Nocardia farcinica | CoA + N-acetyl-2-aminofluorene | - |
? | |
acetyl-CoA + 4-aminobenzoic acid | - |
Nocardia farcinica | CoA + N-acetyl-4-aminobenzoic acid | - |
? | |
acetyl-CoA + 4-aminobenzoic acid | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + 4-aminobiphenyl | - |
Nocardia farcinica | CoA + N-acetyl-4-aminobiphenyl | - |
? | |
acetyl-CoA + 4-aminofluorene | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + 4-aminosalicylate | - |
Nocardia farcinica | CoA + N-acetyl-4-aminosalicylate | - |
? | |
acetyl-CoA + 4-aminosalicylate | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + 4-aminoveratrole | - |
Nocardia farcinica | CoA + N-acetyl-4-aminoveratrole | - |
? | |
acetyl-CoA + 4-aminoveratrole | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + 5-aminosalicylate | - |
Nocardia farcinica | CoA + N-acetyl-5-aminosalicylate | - |
? | |
acetyl-CoA + 5-aminosalicylate | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + benzidine | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + benzidine | - |
Nocardia farcinica | CoA + N-acetyl-benzidine | - |
? | |
acetyl-CoA + beta-naphthylamine | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + beta-naphthylamine | - |
Nocardia farcinica | CoA + N-acetyl-beta-naphthylamine | - |
? | |
acetyl-CoA + hydralazine | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + hydralazine | - |
Nocardia farcinica | CoA + N-acetyl-hydralazine | - |
? | |
acetyl-CoA + isoniazid | - |
Nocardia farcinica | CoA + N-acetyl-isoniazid | - |
? | |
acetyl-CoA + isoniazid | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + sulfamethazine | - |
Nocardia farcinica | ? | - |
? | |
acetyl-CoA + sulfamethazine | - |
Nocardia farcinica | CoA + N-acetyl-sulfamethazine | - |
? | |
acetyl-CoA + sulfamethoxazole | - |
Nocardia farcinica | CoA + N-acetyl-sulfamethoxazole | - |
? | |
acetyl-CoA + sulfamethoxazole | - |
Nocardia farcinica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arylamine N-acetyltransferase | - |
Nocardia farcinica |
NAT | - |
Nocardia farcinica |
NfNAT | - |
Nocardia farcinica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nocardia farcinica |
37 | - |
activity assay | Nocardia farcinica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nocardia farcinica |
7.5 | - |
activity assay | Nocardia farcinica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Nocardia farcinica |