Cloned (Comment) | Organism |
---|---|
into the pKK223-3 bacterial expression vector for transformation of Escherichia coli JM105 cells | Homo sapiens |
isozyme NAT2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in Escherichia coli strain JM105 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several insertion and deletion mutants of NAT2, overview | Homo sapiens |
additional information | ANDH, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | GG, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | GGSG, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | GRSG, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | HHEH, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | PHAG, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | QEGST, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
additional information | SDGSD, human NAT2 mutant, a 17-residue segment is deleted and replaced | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an arylamine | Homo sapiens | the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs | CoA + an N-acetylarylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P11245 | isozyme NAT2; isozyme NAT2 | - |
Homo sapiens | P18440 | isozyme NAT1; isozyme NAT1 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine | structure-function analysis, Cys68-His107-Asp122 catalytic triad | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | wide tissue distribution | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
mutant GGSG | Homo sapiens |
0.0005 | - |
mutant HHEH | Homo sapiens |
0.0006 | - |
mutant GGSG | Homo sapiens |
0.0006 | - |
mutant HHEH | Homo sapiens |
0.0013 | - |
mutant GRSG | Homo sapiens |
0.0014 | - |
mutant GRSG | Homo sapiens |
0.0055 | - |
mutant GG | Homo sapiens |
0.006 | - |
mutant GG | Homo sapiens |
0.0061 | - |
- |
Homo sapiens |
0.0064 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an arylamine | the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs | Homo sapiens | CoA + an N-acetylarylamine | - |
? | |
acetyl-CoA + an arylamine | the enzyme catalyzes the N-acetylation of arylamines and hydrazines and O-acetylation of N-hydroxy-arylamines and heterocyclic amines | Homo sapiens | CoA + an N-acetylarylamine | - |
? | |
acetyl-CoA + sulfamethazine | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structures of wild-type and mutant enzymes, structure-function analysis, computational docking of NAT substrates, NAT homology modeling based on crystal structures of bacteria with Protein Data Bank accession numbers 1E2T, 1W4T, 1GX3, and 2BSZ, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
arylamine N-acetyltransferase | - |
Homo sapiens |
NAT | - |
Homo sapiens |
NAT2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
activity assay | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | control | Homo sapiens |
30 | 33 | mutant GG | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |