Cloned (Comment) | Organism |
---|---|
gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | I6YG32 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | I6YG32 | - |
- |
Mycobacterium tuberculosis H37Rv | I6YG32 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1300 | - |
about, purified recombinant RimI, NatE substrate N-terminal L-methionyl-[ARYFRR], pH 8.0, 25°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-terminal L-methionyl-[ARYFRR] | DP9 peptide (MARYFRR) is a substrate of NatE, a synthetic peptide | Mycobacterium tuberculosis | CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[ARYFRR] | - |
ir | |
additional information | analysis of substrate preference of RimIMtb: substrate peptide DPC (NatA substrate) is custom synthesized with single residue modifications at its N-terminus to represent substrate specificities of NatE (DP9), NatB (DP10), NatC (DP11), and substrate Leu (DP8) and tested, all the peptides are modified by RimIMtb, substrates and sequences, detailed overview. RimIMtb does acetylate peptides representing N-terminus of GroES, GroEL1, and TsaD proteins, in vitro. Significant specific activity of RimIMtb is observed gainst peptide representing N-terminus of GroES. RimIMtb acetylates DP9 (NatE substrate) 2.1fold better than DPC (NatA substrate). RimIMtb acetylates N-terminus of ribosomal proteins and of neighboring non-ribosomal proteins | Mycobacterium tuberculosis | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Nalpha-acetyltransferase | - |
Mycobacterium tuberculosis |
NatE | - |
Mycobacterium tuberculosis |
RimI | - |
Mycobacterium tuberculosis |
RimI acetyltransferase | - |
Mycobacterium tuberculosis |
Rv3420c | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | Nalpha-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nalpha-acetyltransferases (NATs) | Mycobacterium tuberculosis |