Crystallization (Comment) | Organism |
---|---|
enzyme in binary complex with acetyl-CoA, and in ternary complex with CoA and an N-terminal histone H4/H2A, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 200 mM sodium citrate tribasic dihydrate, 100 mM citric acid, pH 4.0 or 20% (w/) PEG 3350 and 200 mM sodium malonate, pH 6.0 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D127A/E129A | the mutant shows significantly decreased catalytic efficiency (1.6-18fold) compared to the wild type enzyme | Homo sapiens |
E139Q | inactive | Homo sapiens |
T174A | the mutant shows wild type catalytic efficiency | Homo sapiens |
W90A | the mutant exhibits markedly decreased catalytic efficiency (2.8fold) compared to the wild type enzyme | Homo sapiens |
Y136A | the mutant shows significantly decreased catalytic efficiency (1.6-1.8fold) compared to the wild type enzyme | Homo sapiens |
Y136F | the catalytic efficiency of the mutant is modestly affected compared to the wild type enzyme | Homo sapiens |
Y138A | the mutant shows significantly decreased catalytic efficiency (1.6-1.8fold) compared to the wild type enzyme | Homo sapiens |
Y211A | the mutant shows wild type catalytic efficiency | Homo sapiens |
Y85A | the mutant exhibits markedly decreased catalytic efficiency (5.8fold) compared to the wild type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal-L-seryl-[histone H2A] | Homo sapiens | - |
an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA | - |
? | |
acetyl-CoA + an N-terminal-L-seryl-[histone H4] | Homo sapiens | - |
an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q86UY6 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel resin column chromatography and HiTrap Q column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + an N-terminal-L-seryl-[histone H2A] | - |
Homo sapiens | an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA | - |
? | |
acetyl-CoA + an N-terminal-L-seryl-[histone H4] | - |
Homo sapiens | an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Naa40p | - |
Homo sapiens |
NAT4 | - |
Homo sapiens |
NatD | - |
Homo sapiens |
Patt1 | - |
Homo sapiens |