Cloned (Comment) | Organism |
---|---|
gene CERS5, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS5 overexpression significantly increases apoptosis in a time- and dose-dependent manner compared to empty vector-transfected controls. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells | Homo sapiens |
gene CERS6, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | CerS6 knockdown by shRNA | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | nonlinear fitting and Michaelis-Menten kinetic analyses | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Homo sapiens | 31966 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine + palmitoyl-CoA | Homo sapiens | - |
N-palmitoylsphinganine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6ZMG9 | - |
- |
Homo sapiens | Q8N5B7 | - |
- |
Purification (Comment) | Organism |
---|---|
subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphinganine + palmitoyl-CoA | - |
Homo sapiens | N-palmitoylsphinganine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthase 5 | - |
Homo sapiens |
ceramide synthase 6 | - |
Homo sapiens |
CerS5 | - |
Homo sapiens |
CerS6 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | ionizing radiation (IR) induces de novo synthesis of ceramide by specifically activating CerS isoforms 2, 5, and 6 | up |
General Information | Comment | Organism |
---|---|---|
malfunction | CerS6 knockdown might decrease apoptosis compared to normal irradiated HeLa cells | Homo sapiens |
malfunction | overexpression of CerS5 increases apoptosis in HeLa cells. CerS2 and CerS5 overexpression significantly alters apoptosis | Homo sapiens |
metabolism | regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type- and subcellular compartment-specific manner. CerS2 and CerS5 overexpression significantly alters apoptosis, determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis | Homo sapiens |
metabolism | regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type- and subcellular compartment-specific manner. CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. Determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview | Homo sapiens |
physiological function | selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozymes CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for IR-induced activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis | Homo sapiens |
physiological function | selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozymes CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for IR-induced activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells | Homo sapiens |