Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | phenylacetyl-CoA partially protects phenylacetyltransferase against 5,5'-dithiobis(2-nitrobenzoate) inactivation | Bos taurus | |
acylglycine | noncompetitive | Bos taurus | |
benzoyl-CoA | - |
Bos taurus | |
butyryl-CoA | - |
Bos taurus | |
CoA | noncompetitve | Bos taurus | |
Ni2+ | 2 mM, 28% residual activity | Bos taurus | |
p-chloromercuribenzoate | - |
Bos taurus | |
Zn2+ | 1 mM, 46% residual activity | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of monovalent cations is required | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phenylacetyl-CoA + glycine = CoA + phenylacetylglycine | sequential reaction mechanism in which the acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves, and the peptide product dissociates last | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.051 | - |
pH not specified in the publication, temperature not specified in the publication | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indoleacetyl-CoA + glycine | - |
Bos taurus | N-indoleacetylglycine + CoA | - |
? | |
phenylacetyl-CoA + glycine | glycine is the preferred acceptor | Bos taurus | phenylacetylglycine + CoA | - |
? | |
phenylacetyl-CoA + L-asparagine | stereospecific for the L-isomer of asparagine | Bos taurus | ? + CoA | - |
? | |
phenylacetyl-CoA + L-glutamine | stereospecific for the L-isomer of glutamine | Bos taurus | ? + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.6 | - |
Bos taurus |