Cloned (Comment) | Organism |
---|---|
gene OsHCT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as soluble GST-tagged enzyme using vector pGEX 5X-1 in Escherichia coli strain BL21(DE3) | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0601 | - |
4-coumaroyl-CoA | pH 7.5, 37°C, with glycerol | Oryza sativa | |
0.0721 | - |
feruloyl-CoA | pH 7.5, 37°C, with glycerol | Oryza sativa | |
0.0859 | - |
glycerol | pH 7.5, 37°C, with feruloyl-CoA | Oryza sativa | |
0.102 | - |
caffeoyl-CoA | pH 7.5, 37°C, with glycerol | Oryza sativa | |
0.1558 | - |
shikimate | pH 7.5, 37°C, with feruloyl-CoA | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryza sativa | no determination of the in vivo substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
gene OsHCT4 | - |
Purification (Comment) | Organism |
---|---|
soluble recombinant GST-tagged OsHCT4 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + glycerol | - |
Oryza sativa | CoA + 2-O-(4-coumaroyl)glycerol + 1-O-(4-coumaroyl)glycerol | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Oryza sativa | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + glycerol | - |
Oryza sativa | CoA + ? | - |
? | |
caffeoyl-CoA + shikimate | - |
Oryza sativa | CoA + 5-O-caffeoylshikimate | - |
? | |
feruloyl-CoA + glycerol | - |
Oryza sativa | CoA + ? | - |
? | |
feruloyl-CoA + shikimate | - |
Oryza sativa | CoA + 5-O-feruloylshikimate | - |
? | |
additional information | no determination of the in vivo substrate | Oryza sativa | ? | - |
? | |
additional information | OsHCT4 mediates the trans-esterification of glycerol as well as shikimic acid in the presence of hydroxycinnamoyl-CoA in vitro, reaction product structure determination by NMR | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HCS/QT | - |
Oryza sativa |
hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase | - |
Oryza sativa |
OsHCT4 | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
evolution | OsHCT1 and OsHCT2 are members of the HCS/QT family, while OsHCT3 and OsHCT4 belong to a distinct group. OsHCT3 and OsHCT4 may employ a different acyl group acceptor | Oryza sativa |