Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-diaminobutyrate | - |
Escherichia coli | |
arsenate | competitive vs. carbamoylphosphate, noncompetitive vs. ornithine | Escherichia coli | |
glycine | - |
Escherichia coli | |
L-2,4-diaminobutyric acid | - |
Escherichia coli | |
L-alanine | - |
Escherichia coli | |
L-asparagine | weak | Escherichia coli | |
L-isoleucine | - |
Escherichia coli | |
L-leucine | - |
Escherichia coli | |
L-lysine | weak | Escherichia coli | |
L-norvaline | - |
Escherichia coli | |
L-serine | weak | Escherichia coli | |
L-valine | - |
Escherichia coli | |
methionine | L-isomer | Escherichia coli | |
ornithine | at high concentrations; noncompetitive substrate inhibition vs. carbamoylphosphate | Escherichia coli | |
phosphate | competitive vs. carbamoylphosphate, noncompetitive vs. ornithine | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
3 * 35000, SDS-PAGE | Escherichia coli |
105000 | - |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | Escherichia coli | enzyme belongs to the arginine biosynthesis pathway | phosphate + L-citrulline | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat, ammonium sulfate, DEAE-Sephadex, Sephadex G-200, aminohexyl-Sepharose | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline | mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2833 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Escherichia coli | phosphate + L-citrulline | - |
r | |
carbamoyl phosphate + L-ornithine | enzyme belongs to the arginine biosynthesis pathway | Escherichia coli | phosphate + L-citrulline | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 35000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
half-life: 5 min, 8 min with 500 mM KCl, 10 min with 10 mM citrulline, 20 min with 100 mM citrulline, 43 min with 20 mM phosphate, 48 min with 100 mM ornithine, 108 min with 100 mM phosphate, 120 min with 10 mM phosphate and 10 mM ornithine, 122 min with 10 mM phosphate and 100 mM citrulline, more than 200 min with 100 mM phosphate and 100 mM citrulline or with 10 mM phosphate and 100 mM ornithine | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-norvaline | - |
Escherichia coli | |
5.5 | - |
L-leucine | - |
Escherichia coli | |
7 | - |
2,4-diaminobutyrate | - |
Escherichia coli | |
13 | - |
L-alanine | - |
Escherichia coli | |
13.5 | - |
L-valine | - |
Escherichia coli | |
14 | - |
L-isoleucine | - |
Escherichia coli | |
15 | - |
glycine | - |
Escherichia coli | |
40 | - |
L-methionine | - |
Escherichia coli | |
63.5 | - |
L-serine | - |
Escherichia coli | |
110 | - |
L-asparagine | - |
Escherichia coli |