Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K239A | the mutant shows no activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | almost complete inhibition at 100 mM | Homo sapiens | |
S-adenosyl-L-homocysteine | inhibitory at concentrations beyond 0.002 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme exhibits optimum activity in the presence of 2 mM MgCl2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid resin column chromatography and heparin-Sepharose 6 column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes specific methylation of the 2'-O-ribose of the first nucleotide of a capped RNA transcript | Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppG-RNA | cap1 formation is optimal at 0.005 mM S-adenosyl-L-methionine | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppGm-RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cap1 2-O'-ribose methyltransferase | - |
Homo sapiens |
FtsJ methyltransferase domain-containing 2 | - |
Homo sapiens |
FTSJD2 | - |
Homo sapiens |
ISG95 | - |
Homo sapiens |
Mtr1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
about pH 7.0 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is responsible for cap1 formation in vivo | Homo sapiens |