Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Linum usitatissimum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme downregulation leads to reduced lignin quantity a modified syringyl/guaiacyl lignin monomer ratio in the non-condensed lignin fraction, together with altered xylem organization, reduced cell-wall thickness and the appearance of an irregular xylem phenotype, phentypes of two independently down-regulated lines, overview | Linum usitatissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
caffeoyl-CoA | recombinant enzyme, 30°C | Linum usitatissimum | |
0.039 | - |
caffeic acid | recombinant enzyme, 30°C | Linum usitatissimum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27900 | - |
x * 27900, about, sequence calculation | Linum usitatissimum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Linum usitatissimum | CCoAOMT down-regulation induces changes in xylem cell-wall structure and the lignin fractions | ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | Linum usitatissimum | - |
S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeic acid | Linum usitatissimum | - |
S-adenosyl-L-homocysteine + ferulic acid | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | Linum usitatissimum | - |
S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Linum usitatissimum | Q4JHB1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag | Linum usitatissimum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Linum usitatissimum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CCoAOMT down-regulation induces changes in xylem cell-wall structure and the lignin fractions | Linum usitatissimum | ? | - |
? | |
additional information | flax CCoAOMT possesses a small, but probably significant 5' methylating activity, in addition to a more usual 3' methylating activity | Linum usitatissimum | ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | - |
Linum usitatissimum | S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA | 3' methylating activity | Linum usitatissimum | S-adenosyl-L-homocysteine + sinapoyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeic acid | - |
Linum usitatissimum | S-adenosyl-L-homocysteine + ferulic acid | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | - |
Linum usitatissimum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? | |
S-adenosyl-L-methionine + caffeoyl-CoA | preferred substrate, 3' methylating activity | Linum usitatissimum | S-adenosyl-L-homocysteine + feruloyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27900, about, sequence calculation | Linum usitatissimum |
Synonyms | Comment | Organism |
---|---|---|
caffeoyl coenzyme A O-methyltransferase | - |
Linum usitatissimum |
CCoAOMT | - |
Linum usitatissimum |
More | the enzyme belongs to the O-methyltransferase superfamily | Linum usitatissimum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Linum usitatissimum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Linum usitatissimum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Linum usitatissimum | sequence calculation | - |
5.3 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes central essential reactions in the lignin biosynthesis, pathway overview | Linum usitatissimum |