Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutaredoxin | required, reverses enzyme inhibition by oxidized glutathione, glutathione together with glutaredoxin exhibits regulatory function in vivo | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant lacking glutathione reductase and glutaredoxins does barely grow on sulfate, strains containing a mutant C9S/C12S glutaredoxin grow poorly | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxidized glutathione | reversible inhibition, forms mixed disulfides with the enzymes active site residue Cys239, inactivation kinetics, inhibition is reversible by glutaredoxin, not by DTT, glutaredoxin together with glutathione exhibits regulatory function in vivo | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl-sulfate + glutaredoxin | Escherichia coli | - |
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | Escherichia coli | - |
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
additional information | Escherichia coli | enzyme is involved in sulfate assimilation requiring reduced glutathione and glutaredoxins, redox regulation of the enzyme by glutathione and glutaredoxins, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain BL21(DE3) | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin | ping pong type reaction mechanism, Cys239 is the active site residue | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl-sulfate + glutaredoxin | - |
Escherichia coli | adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite | - |
? | |
3'-phosphoadenylyl-sulfate + thioredoxin | - |
Escherichia coli | adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite | - |
? | |
additional information | enzyme is involved in sulfate assimilation requiring reduced glutathione and glutaredoxins, redox regulation of the enzyme by glutathione and glutaredoxins, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3'-Phosphoadenylylsulfate reductase | - |
Escherichia coli |
PAPS reductase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutaredoxin | - |
Escherichia coli | |
thioredoxin | - |
Escherichia coli |