Protein Variants | Comment | Organism |
---|---|---|
C239S | mutant enzymes Cys239Ser and Tyr209Phe are inactive | Escherichia coli |
Q160L | mutant enzyme Gln160Leu is as active as the wild-type enzyme | Escherichia coli |
Y209F | mutant enzymes Cys239Ser and Tyr209Phe are inactive | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-bisphosphate | competitive with respect to 5-phosphoadenosine 3-phosphosulfate | Escherichia coli | |
N-(Iodoacetyl-aminoethyl)-5-N'-naphthylamin-1-sulfonic acid | - |
Escherichia coli | |
N-bromosuccinimide | - |
Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5-Phosphoadenosine 3-phosphosulfate | - |
Escherichia coli | |
0.023 | - |
reduced thioredoxin | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
recombinant enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin | special ping-pong mechanism with 5-phosphoadenosine 3-phosphosulfate reacting with the reduced enzyme isomer in a Theorell-Chance mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
99 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin | - |
Escherichia coli | adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite | - |
? |