Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Rhodovulum sulfidophilum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the enzyme contains 3.5 mol Fe per mol enzyme | Rhodovulum sulfidophilum | |
Molybdenum | the enzyme contains 0.5 mol molybdenum per mol enzyme | Rhodovulum sulfidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
38000 | - |
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
94000 | - |
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
152000 | - |
gel filtration | Rhodovulum sulfidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodovulum sulfidophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration | Rhodovulum sulfidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.2 | - |
crude extract, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication | Rhodovulum sulfidophilum |
20.2 | - |
after purification, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication | Rhodovulum sulfidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethyl sulfoxide + reduced 2,6-dichloroindophenol | - |
Rhodovulum sulfidophilum | dimethyl sulfide + oxidized 2,6-dichloroindophenol + H2O | - |
? | |
dimethyl sulfoxide + reduced methyl viologen + H2O | 2% activity compared to trimethylamine N-oxide | Rhodovulum sulfidophilum | dimethyl sulfide + oxidized methyl viologen | - |
? | |
lauryldimethylamine N-oxide + reduced methyl viologen + H2O | 69% activity compared to trimethylamine N-oxide | Rhodovulum sulfidophilum | ? | - |
? | |
additional information | no activity with chlorate and bromate | Rhodovulum sulfidophilum | ? | - |
? | |
trimethylamine N-oxide + reduced methyl viologen + H2O | 100% activity | Rhodovulum sulfidophilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE | Rhodovulum sulfidophilum |
Synonyms | Comment | Organism |
---|---|---|
dimethylsulfide: acceptor oxidoreductase | - |
Rhodovulum sulfidophilum |
Me2S:acceptor oxidoreductase | - |
Rhodovulum sulfidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the cytochrome contains a b-type heme and a content of 0.65 mol protoheme per mol enzyme is determined | Rhodovulum sulfidophilum | |
iron-sulfur centre | - |
Rhodovulum sulfidophilum | |
molybdopterin | - |
Rhodovulum sulfidophilum |