Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfite | 2 mM, 1.6fold stimulation. Inhibitory at higher concentrations, almost complete inhibition at 10 mM | Acidithiobacillus ferrooxidans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acidithiobacillus ferrooxidans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfate | 200 mM, about 60% reduction in activity | Acidithiobacillus ferrooxidans | |
sulfite | 2 mM, 1.6fold stimulation. Inhibitory at higher concentrations, almost complete inhibition at 10 mM | Acidithiobacillus ferrooxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
thiosulfate | recombinant protein, pH 2.5, 50°C | Acidithiobacillus ferrooxidans | |
15 | - |
thiosulfate | native protein, pH 2.5, 50°C | Acidithiobacillus ferrooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE, x * 25796, calculated, mature protein | Acidithiobacillus ferrooxidans |
25796 | - |
x * 25000, SDS-PAGE, x * 25796, calculated, mature protein | Acidithiobacillus ferrooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | B7J3E3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence contains a signal peptide of 37 amino acids | Acidithiobacillus ferrooxidans |
Purification (Comment) | Organism |
---|---|
partial | Acidithiobacillus ferrooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.16 | - |
pH 2.5, 40°C, recombinant protein | Acidithiobacillus ferrooxidans |
6.5 | - |
native protein, pH 2.5, 50°C | Acidithiobacillus ferrooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 thiosulfate + 2 ferricyanide | no catalytic activity below 4 mM thiosulfate. Enzyme catalyzes the formation of 1 mol of tetrathionate from 2 mol of thiosulfate | Acidithiobacillus ferrooxidans | tetrathionate + 2 ferrocyanide | - |
? | |
additional information | no substrates: sulfite, ubiquinone and horse heart cytochrome c | Acidithiobacillus ferrooxidans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE, x * 25796, calculated, mature protein | Acidithiobacillus ferrooxidans |
Synonyms | Comment | Organism |
---|---|---|
AFE_0042 | locus name | Acidithiobacillus ferrooxidans |
Tat pathway signal sequence domain protein | - |
Acidithiobacillus ferrooxidans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant protein | Acidithiobacillus ferrooxidans |
70 | - |
native protein | Acidithiobacillus ferrooxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: heme | Acidithiobacillus ferrooxidans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acidithiobacillus ferrooxidans | calculated, mature protein | - |
6.2 |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme activity is detected in strains grown on tetrathionate or sulfur, but no activity is detected in ferrous iron-grown cells | Acidithiobacillus ferrooxidans |