General Stability | Organism |
---|---|
low ionic strength, 70 h, 0.002 mg protein/ml, 10 mM phosphate buffer or 50 mM Tris-HCl buffer, pH 7.5, unstable | Leptolyngbya boryana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dithionite | converts native enzyme in modified form without ferredoxin-linked activity but retained methyl viologen-linked activity | Leptolyngbya boryana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
reduced ferredoxin | - |
Leptolyngbya boryana | |
2.5 | - |
reduced methyl viologen | - |
Leptolyngbya boryana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | molybdenum iron-sulfur protein | Leptolyngbya boryana | |
Iron | contains 4 iron and 4 acid-labile sulfur atoms per enzyme molecule | Leptolyngbya boryana | |
iron-sulfur centre | contains 4 iron and 4 acid-labile sulfur atoms per enzyme molecule, presence of iron-sulfur cluster | Leptolyngbya boryana | |
Molybdenum | molybdenum iron-sulfur protein | Leptolyngbya boryana | |
Molybdenum | enzyme contains 0.95 atom of molybdenum per enzyme molecule | Leptolyngbya boryana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
1 * 83000, SDS-PAGE | Leptolyngbya boryana |
85000 | - |
sedimentation equilibrium method | Leptolyngbya boryana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced ferredoxin | Leptolyngbya boryana | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
nitrate + reduced ferredoxin | Leptolyngbya boryana | two-electron nitrate reduction | nitrite + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptolyngbya boryana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
19000fold purification | Leptolyngbya boryana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
305 | - |
ferredoxin-linked activity | Leptolyngbya boryana |
1020 | - |
methyl viologen-linked activity | Leptolyngbya boryana |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM Tris-HCl buffer, pH 7.5, 0.5 M NaCl, 1 mM EDTA, 1 month, no loss of activity | Leptolyngbya boryana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced ferredoxin | - |
Leptolyngbya boryana | nitrite + H2O + oxidized ferredoxin | - |
? | |
nitrate + reduced ferredoxin | assimilatory nitrate reduction | Leptolyngbya boryana | nitrite + oxidized ferredoxin | - |
? | |
nitrate + reduced ferredoxin | two-electron nitrate reduction | Leptolyngbya boryana | nitrite + oxidized ferredoxin | - |
? | |
nitrate + reduced methyl viologen | enzyme also has methyl viologen-linked activity | Leptolyngbya boryana | nitrite + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 83000, SDS-PAGE | Leptolyngbya boryana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leptolyngbya boryana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
anaerobic assay, methyl viologen- and ferredoxin-linked activity | Leptolyngbya boryana |
10.2 | - |
aerobic assay, methyl viologen-linked activity | Leptolyngbya boryana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9.2 | at pH 7.3 and 9.2: 50% of activity maximum, aerobic assay, methyl viologen-linked and ferredoxin-linked activity | Leptolyngbya boryana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | contains 4 iron and 4 acid-labile sulfur atoms per enzyme molecule, presence of iron-sulfur cluster | Leptolyngbya boryana |