Cloned (Comment) | Organism |
---|---|
expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein | Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of 541 residue amonio-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
nitrate | His-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea | |
0.012 | - |
nitrate | GST-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | Mo/protein ratio 0.44 for His-tagged protein, 0.93 for GST-tagged protein | Spinacia oleracea | |
additional information | tungsten cannot replace for molybdenum in the GST-tagged protein | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain | Spinacia oleracea |
85000 | - |
x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0015 | - |
His-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |
0.0108 | - |
GST-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced methyl viologen + nitrate | - |
Spinacia oleracea | methyl viologen + nitrite | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE, His-tagged domain, x * 85000, SDS-PAGE, GST-tagged domain | Spinacia oleracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
nitrate | GST-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea | |
2.5 | - |
nitrate | His-tagged molybdenum domain, 25°C, pH 7.6 | Spinacia oleracea |