Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one | - |
Paenarthrobacter nicotinovorans | |
3-(4-aminobutyl)-pyridine | - |
Paenarthrobacter nicotinovorans | |
6-hydroxy-L-nicotine | - |
Paenarthrobacter nicotinovorans | |
D,L-2-Hydroxynicotine | - |
Paenarthrobacter nicotinovorans | |
Phenanthroline | inactivation above 1 mM | Paenarthrobacter nicotinovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
6-hydroxy-D-nicotine | - |
Paenarthrobacter nicotinovorans | |
0.2 | - |
6-amino-D-nicotine | - |
Paenarthrobacter nicotinovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
sedimentation equilibrium and SDS-PAGE | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
additional information | Paenarthrobacter nicotinovorans | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenarthrobacter nicotinovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.5 | - |
- |
Paenarthrobacter nicotinovorans |
Storage Stability | Organism |
---|---|
4°C, fairly stable at neutral or alkaline pH in presence of 10 mM mercaptoethanol | Paenarthrobacter nicotinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-aminonicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(-aminopyridin-3-yl)-4-methylamino-butan-1-one + H2O2 | - |
? | |
(R)-6-hydroxynicotine + H2O + electron acceptor | as electron acceptors methylene blue and 2,6-dichlorophenolindophenol aerobically and anaerobically | Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + electron donor | - |
? | |
(R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
additional information | - |
Paenarthrobacter nicotinovorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Paenarthrobacter nicotinovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
inactivation above this temperature | Paenarthrobacter nicotinovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in 0.1 M Tris-HCl buffer | Paenarthrobacter nicotinovorans |
8.5 | - |
0.1 M glycine-NaOH buffer | Paenarthrobacter nicotinovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
below this pH inactivation | Paenarthrobacter nicotinovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one mol FAD per mol enzyme | Paenarthrobacter nicotinovorans | |
FAD | covalently bound to a histidine | Paenarthrobacter nicotinovorans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one | - |
Paenarthrobacter nicotinovorans | |
0.7 | - |
3-(4-aminobutyl)-pyridine | - |
Paenarthrobacter nicotinovorans | |
1.5 | - |
6-hydroxy-L-nicotine | - |
Paenarthrobacter nicotinovorans | |
1.7 | - |
D,L-2-Hydroxynicotine | - |
Paenarthrobacter nicotinovorans |