Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli S30 wild type and mutants | Paenarthrobacter nicotinovorans |
Protein Variants | Comment | Organism |
---|---|---|
H71C | only residual enzyme activity | Paenarthrobacter nicotinovorans |
P73A | unable to bind FAD | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD is bound via an (8alpha)-isoalloxazine-(N3)histidyl linkage | Paenarthrobacter nicotinovorans |