Literature summary for 1.5.1.3 extracted from

Babu, C.S.; Lim, C.
Influence of solution ionic strength on the stabilities of M20 loop conformations in apo E. coli dihydrofolate reductase (2021), J. Chem. Phys., 154, 195103 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of protein crystallized in varying ionic strengths. High ionic strengths (0.75/1.5M) can preferentially stabilize the loop in closed/occluded conformations	Escherichia coli

General Stability

General Stability	Organism
at ionic strengths below the intracellular ion concentration-derived ionic strength in Escherichia coli ( at or below 0.237 M), the DHFR M20 loop tends to adopt open/closed conformations, and rarely an occluded loop state. As the ionic strength exceeds the physiological ionic strength of 0.237 M, the loop tends to adopt a closed/occluded conformation. the solution ionic strength affects the M20 loop stability differently depending on its conformation: High ionic strengths stabilize the occluded conformation more than low ionic strengths, as Ca2+ ions can approach E17 of the M20 loop and stabilize its orientation, whereas they are distal from the E17 at low ionic strengths. Both low and high ionic strengths can stabilize the closed conformation,	Escherichia coli
but they do not significantly affect the stability of the open conformation	Escherichia coli

Organism

at ionic strengths below the intracellular ion concentration-derived ionic strength in Escherichia coli ( at or below 0.237 M), the DHFR M20 loop tends to adopt open/closed conformations, and rarely an occluded loop state. As the ionic strength exceeds the physiological ionic strength of 0.237 M, the loop tends to adopt a closed/occluded conformation. the solution ionic strength affects the M20 loop stability differently depending on its conformation: High ionic strengths stabilize the occluded conformation more than low ionic strengths, as Ca2+ ions can approach E17 of the M20 loop and stabilize its orientation, whereas they are distal from the E17 at low ionic strengths. Both low and high ionic strengths can stabilize the closed conformation,

Escherichia coli

but they do not significantly affect the stability of the open conformation

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0ABQ4	-	-

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Release 2023.1 (January 2023)