Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces diastatochromogenes |
General Stability | Organism |
---|---|
the 50% thermal inactivation temperature of the proteinase K treated enzyme is increased from 50 to 70°C | Streptomyces diastatochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
L-glutamate | proteinase K treated enzyme, at pH 8.0 and 30°C | Streptomyces diastatochromogenes | |
0.66 | - |
L-glutamate | recombinant enzyme, at pH 8.0 and 30°C | Streptomyces diastatochromogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | Streptomyces diastatochromogenes | the enzyme exhibits strict specificity for L-glutamate | 2-oxoglutarate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces diastatochromogenes | A0A1B1PF34 | - |
- |
Purification (Comment) | Organism |
---|---|
His trap Ni-NTA column chromatography | Streptomyces diastatochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | the enzyme exhibits strict specificity for L-glutamate | Streptomyces diastatochromogenes | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamine + O2 + H2O | 0.63% activity compared to L-glutamate | Streptomyces diastatochromogenes | 2-oxoglutaramate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LGOX | - |
Streptomyces diastatochromogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces diastatochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the 50% thermal inactivation temperature of recombinant enzyme is about 50°C | Streptomyces diastatochromogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces diastatochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptomyces diastatochromogenes |