Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli JM109 cells | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
H312A | the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids | Streptomyces sp. |
R305A | the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-histidine) | Streptomyces sp. |
R305D | the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-arginine) | Streptomyces sp. |
R305K | the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-histidine) | Streptomyces sp. |
R305L | the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-histidine) | Streptomyces sp. |
W564A | the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.173 | - |
L-glutamate | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | Streptomyces sp. | specific substrate | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
additional information | Streptomyces sp. | no activity with L-Leu, L-Phe, L-Asn, L-His, and L-Arg | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | Q8L3C7 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE Toyopearl 650 column chromatography | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | specific substrate | Streptomyces sp. | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
additional information | no activity with L-Leu, L-Phe, L-Asn, L-His, and L-Arg | Streptomyces sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LGOX | - |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
53.2 | - |
L-glutamate | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
308 | - |
L-glutamate | wild type enzyme, pH and temperature not specified in the publication | Streptomyces sp. |