Application | Comment | Organism |
---|---|---|
medicine | recombinant l-GlOx may be used for food analysis or in the monitoring of L-glutamate as a neurotransmitter, can be used to construct micro sensors and microanalysis systems, performs very similarly to the commercially available wild-type l-GlOx, the recombinant l-GlOx is suitable for mass production | Streptomyces sp. |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli JM109 | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
1 * 40000, 1 * 17000, and 1 * 12000, SDSPAGE, recombinant enzyme | Streptomyces sp. |
17000 | - |
1 * 40000, 1 * 17000, and 1 * 12000, SDSPAGE, recombinant enzyme | Streptomyces sp. |
40000 | - |
1 * 40000, 1 * 17000, and 1 * 12000, SDSPAGE, recombinant enzyme | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + O2 + H2O | slight catalytic activity with the micro L-glutamate sensor with recombinant l-GlOx | Streptomyces sp. | ? | - |
? | |
L-aspartate + O2 + H2O | slight catalytic activity with the micro L-glutamate sensor with recombinant l-GlOx | Streptomyces sp. | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamate + O2 + H2O | - |
Streptomyces sp. | 2-oxoglutarate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 40000, 1 * 17000, and 1 * 12000, SDSPAGE, recombinant enzyme | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
l-GlOx | - |
Streptomyces sp. |