Literature summary for 1.4.3.1 extracted from

Molla, G.; Chaves-Sanjuan, A.; Savinelli, A.; Nardini, M.; Pollegioni, L.
Structure and kinetic properties of human D-aspartate oxidase, the enzyme-controlling D-aspartate levels in brain (2020), FASEB J., 34, 1182-1197 .

Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of mutants C141Y and C143G, to 3.22 A resolution. The FAD-binding domain is comprised of residues 1-46, 143-192, 282-335 with the canonical dinucleotide binding fold observed in several flavoenzymes, and the substrate-binding domain (residues 47-142, 193-281) is characterized by a large, eight-stranded, mixed beta-sheet	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C141Y	variant produces crystals suitable for X-ray analysis	Homo sapiens
C143G	variant produces crystals suitable for X-ray analysis	Homo sapiens
H54A	variant shows a lower maximal activity and kinetic efficiency compared to the wild-type	Homo sapiens
R237A	variant shows a lower maximal activity and kinetic efficiency compared to the wild-type	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.34	-	O2	wild-type, pH 8.3, 25°C	Homo sapiens
1.05	-	D-Aspartate	wild-type, pH 8.3, 25°C	Homo sapiens
2.76	-	N-Methyl-D-aspartate	wild-type, pH 8.3, 25°C	Homo sapiens
31.5	-	D-glutamate	wild-type, pH 8.3, 25°C	Homo sapiens
67	-	D-asparagine	wild-type, pH 8.3, 25°C	Homo sapiens
96	-	D-histidine	wild-type, pH 8.3, 25°C	Homo sapiens
339	-	D-proline	wild-type, pH 8.3, 25°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39200	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q99489	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-asparagine + H2O + O2	-	Homo sapiens	2-oxosuccinamic acid + H2O2 + NH3	-	?
D-aspartate + H2O + O2	-	Homo sapiens	oxaloacetate + NH3 + H2O2	-	?
D-glutamate + H2O + O2	-	Homo sapiens	2-oxoglutarate + NH3 + H2O2	-	?
D-histidine + H2O + O2	-	Homo sapiens	3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2	-	?
D-proline + H2O + O2	-	Homo sapiens	? + H2O2 + NH3	-	?
N-methyl-D-aspartate + H2O + O2	-	Homo sapiens	oxaloacetate + CH3NH2 + H2O2	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 38647, calculated from sequence	Homo sapiens

Synonyms

Synonyms	Comment	Organism
DASPO	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
48.8	-	melting temperature	Homo sapiens
52.5	-	melting temperature, presence of FAD	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.2	-	D-proline	wild-type, pH 8.3, 25°C	Homo sapiens
1.2	-	D-histidine	wild-type, pH 8.3, 25°C	Homo sapiens
8.3	-	D-asparagine	wild-type, pH 8.3, 25°C	Homo sapiens
11.3	-	D-glutamate	wild-type, pH 8.3, 25°C	Homo sapiens
73.6	-	N-Methyl-D-aspartate	wild-type, pH 8.3, 25°C	Homo sapiens
81.3	-	D-Aspartate	wild-type, pH 8.3, 25°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	the flavin ring is located at the interface between the two protein domains, with nearly all hydrogen-bond donors and acceptors of FAD interacting with protein atoms	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	the reductive half-reaction is reversible. The kinetic mechanism follows a ternary complex mechanism characterized by a very fast step of flavin reduction followed by a rate-limiting reoxidation step	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.003	-	D-proline	wild-type, pH 8.3, 25°C	Homo sapiens
0.011	-	D-histidine	wild-type, pH 8.3, 25°C	Homo sapiens
0.12	-	D-asparagine	wild-type, pH 8.3, 25°C	Homo sapiens
0.36	-	D-glutamate	wild-type, pH 8.3, 25°C	Homo sapiens
26.7	-	N-Methyl-D-aspartate	wild-type, pH 8.3, 25°C	Homo sapiens
77.4	-	D-Aspartate	wild-type, pH 8.3, 25°C	Homo sapiens

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Release 2023.1 (January 2023)