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Literature summary for 1.4.3.1 extracted from

  • Katane, M.; Kuwabara, H.; Nakayama, K.; Saitoh, Y.; Miyamoto, T.; Sekine, M.; Homma, H.
    Rat D-aspartate oxidase is more similar to the human enzyme than the mouse enzyme (2018), Biochim. Biophys. Acta, 1866, 806-812 .
    View publication on PubMed
Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus
expression in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5-aminonicotinic acid directly binds to the active site Mus musculus
5-aminonicotinic acid directly binds to the active site Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
146000
-
 PAGE Mus musculus
146000
-
 PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Mus musculus Q0E9Q7
-
-
Rattus norvegicus D3ZDM7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
 substrate D-glutamate, pH 8.3, 37°C Mus musculus
1.77
-
 substrate D-glutamate, pH 8.3, 37°C Rattus norvegicus
11.7
-
 substrate D-aspartate, pH 8.3, 37°C Mus musculus
51.7
-
 substrate D-aspartate, pH 8.3, 37°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aspartate + H2O + O2
-
 Mus musculus oxaloacetate + H2O2 + NH3
-
 ?
D-aspartate + H2O + O2
-
 Rattus norvegicus oxaloacetate + H2O2 + NH3
-
 ?
D-glutamate + H2O + O2
-
 Mus musculus 2-oxoglutarate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2
-
 Rattus norvegicus 2-oxoglutarate + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41700, calculated from sequence, His-tagged protein Mus musculus
tetramer 4 * 41700, calculated from sequence, His-tagged protein Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Mus musculus
45
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
8.3 12.5
-
 Rattus norvegicus
8.3 11
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Mus musculus
FAD Kd value 0.128 microM Rattus norvegicus