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Literature summary for 1.4.3.1 extracted from

  • Takahashi, S.; Osugi, K.; Shimekake, Y.; Shinbo, A.; Abe, K.; Kera, Y.
    Characterization and improvement of substrate-binding affinity of D-aspartate oxidase of the thermophilic fungus Thermomyces dupontii (2019), Appl. Microbiol. Biotechnol., 103, 4053-4064 .
    View publication on PubMed
Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermomyces dupontii

Protein Variants

Protein Variants Comment Organism
F248Y mutation markedly increases substrate-binding affinity Thermomyces dupontii

General Stability

General Stability Organism
enzyme stability decreases following a decrease in protein concentration, and externally added FAD cannot repress the destabilization Thermomyces dupontii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.71
-
 D-glutamate mutant F248Y, pH 7, 60°C Thermomyces dupontii
0.87
-
 D-Aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
2.16
-
 D-glutamate wild-type, pH 7, 60°C Thermomyces dupontii
6.7
-
 N-Methyl-D-aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
8.77
-
 D-Aspartate wild-type, pH 7, 60°C Thermomyces dupontii
25.7
-
 D-glutamine mutant F248Y, pH 7, 60°C Thermomyces dupontii
103
-
 N-Methyl-D-aspartate wild-type, pH 7, 60°C Thermomyces dupontii
108
-
 D-glutamine wild-type, pH 7, 60°C Thermomyces dupontii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38300
-
 gel filtration Thermomyces dupontii

Organism

Organism UniProt Comment Textmining
Thermomyces dupontii
-
-
-
Thermomyces dupontii NBRC 30541
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.3
-
 37°C, pH 7.5 Thermomyces dupontii
169
-
 60°C, pH 7.5 Thermomyces dupontii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aspartate + H2O + O2
-
 Thermomyces dupontii oxaloacetate + H2O2 + NH3
-
 ?
D-aspartate + H2O + O2
-
 Thermomyces dupontii NBRC 30541 oxaloacetate + H2O2 + NH3
-
 ?
D-glutamate + H2O + O2
-
 Thermomyces dupontii 2-oxoglutarate + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2
-
 Thermomyces dupontii NBRC 30541 2-oxoglutarate + NH3 + H2O2
-
 ?
D-glutamine + H2O + O2
-
 Thermomyces dupontii 2-oxoglutaric acid + H2O2 + NH3
-
 ?
D-glutamine + H2O + O2
-
 Thermomyces dupontii NBRC 30541 2-oxoglutaric acid + H2O2 + NH3
-
 ?
N-methyl-D-aspartate + H2O + O2
-
 Thermomyces dupontii oxaloacetate + CH3NH2 + H2O2
-
 ?
N-methyl-D-aspartate + H2O + O2
-
 Thermomyces dupontii NBRC 30541 oxaloacetate + CH3NH2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Thermomyces dupontii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermomyces dupontii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
51
-
 T50 value Thermomyces dupontii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39.9
-
 D-glutamine mutant F248Y, pH 7, 60°C Thermomyces dupontii
50.9
-
 D-Aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
58.1
-
 D-glutamate mutant F248Y, pH 7, 60°C Thermomyces dupontii
68.9
-
 N-Methyl-D-aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
123
-
 D-Aspartate wild-type, pH 7, 60°C Thermomyces dupontii
144
-
 D-glutamine wild-type, pH 7, 60°C Thermomyces dupontii
155
-
 N-Methyl-D-aspartate wild-type, pH 7, 60°C Thermomyces dupontii
217
-
 D-glutamate wild-type, pH 7, 60°C Thermomyces dupontii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermomyces dupontii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10
-
 Thermomyces dupontii

Cofactor

Cofactor Comment Organism Structure
FAD typical absorption spectrum associated with flavoproteins with maxima at 273, 375, and 455 nm Thermomyces dupontii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 D-glutamine wild-type, pH 7, 60°C Thermomyces dupontii
1.5
-
 N-Methyl-D-aspartate wild-type, pH 7, 60°C Thermomyces dupontii
10.2
-
 N-Methyl-D-aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
14
-
 D-Aspartate wild-type, pH 7, 60°C Thermomyces dupontii
15.5
-
 D-glutamine mutant F248Y, pH 7, 60°C Thermomyces dupontii
58.6
-
 D-Aspartate mutant F248Y, pH 7, 60°C Thermomyces dupontii
81
-
 D-glutamate mutant F248Y, pH 7, 60°C Thermomyces dupontii
100
-
 D-glutamate wild-type, pH 7, 60°C Thermomyces dupontii