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Literature summary for 1.4.1.14 extracted from

  • Masters, D.S.; Meister, A.
    Inhibition by homocysteine sulfonamide of glutamate synthase purified from Saccharomyces cerevisiae (1982), J. Biol. Chem., 257, 8711-8715.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid potent irreversible inhibitor Saccharomyces cerevisiae
6-diazo-5-oxo-L-norleucine 0.1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition Saccharomyces cerevisiae
D-glutamine slight inhibition Saccharomyces cerevisiae
DL-ethionine sulfone potent competitive inhibitor Saccharomyces cerevisiae
DL-homocysteic acid
-
Saccharomyces cerevisiae
L-2-Amino-4-oxo-5-chloropentanoate 1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition Saccharomyces cerevisiae
L-Albizziin less inhibitory than L-homocysteine sulfonamide, methionine sulfone and ethionine sulfone Saccharomyces cerevisiae
L-homocysteine sulfonamide very potent transition state inhibitor, competitive to L-glutamine Saccharomyces cerevisiae
L-methionine sulfone very potent competitive inhibitor Saccharomyces cerevisiae
L-methionine-SR-sulfoxide slight inhibition Saccharomyces cerevisiae
O-diazoacetyl-L-serine 1 mM, 30 min, 0°C, pH 7.6, irreversible inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADH
-
Saccharomyces cerevisiae
0.04
-
2-oxoglutarate
-
Saccharomyces cerevisiae
0.28
-
L-glutamine
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol Saccharomyces cerevisiae
169000
-
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol Saccharomyces cerevisiae
265000
-
-
Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
7500fold purification Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH low activity when NH3 is substituted for glutamine, NH4Cl: 2.7% of the activity with L-glutamine Saccharomyces cerevisiae L-glutamate + NAD+
-
?
L-glutamine + 2-oxoglutarate + NADH specific for NADH Saccharomyces cerevisiae L-glutamate + NAD+
-
?
additional information no activity with 2-oxovalerate, 2-oxopimelate, 2-oxoadipate, oxaloacetate, D-glutamine, L- or D-asparagine, L- or D-homoglutamine, alpha-methyl-L-glutamine, DL-beta-glutamine, L-isoglutamine, glutaramide or glutaramate Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 glutamine-dependent NADH oxidase activity Saccharomyces cerevisiae
9
-
ammonia-dependent NADH oxidase activity, rate steadily increases from pH 6 to 9 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Saccharomyces cerevisiae
NADH specific for NADH as reductant Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0036
-
L-homocysteine sulfonamide
-
Saccharomyces cerevisiae
0.005
-
L-methionine sulfone
-
Saccharomyces cerevisiae
0.015
-
DL-ethionine sulfone
-
Saccharomyces cerevisiae
0.1
-
L-Albizziin
-
Saccharomyces cerevisiae
0.146
-
(2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid
-
Saccharomyces cerevisiae
0.178
-
DL-homocysteic acid
-
Saccharomyces cerevisiae
0.3
-
D-glutamine
-
Saccharomyces cerevisiae
0.3
-
L-methionine-SR-sulfoxide
-
Saccharomyces cerevisiae