Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Archaeoglobus fulgidus |
General Stability | Organism |
---|---|
optimal KCl concentration for stability is 1.5 M | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ketobutyrate | - |
Archaeoglobus fulgidus | |
L-2-aminobutyrate | - |
Archaeoglobus fulgidus | |
L-Ala | - |
Archaeoglobus fulgidus | |
NADH | - |
Archaeoglobus fulgidus | |
pyruvate | - |
Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | pH 8.5, 82°C | Archaeoglobus fulgidus | |
0.085 | - |
L-2-aminobutyrate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
0.16 | - |
pyruvate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
0.48 | - |
2-oxobutyrate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
0.6 | - |
NAD+ | pH 8.5, 82°C | Archaeoglobus fulgidus | |
0.71 | - |
L-Ala | pH 8.5, 82°C | Archaeoglobus fulgidus | |
0.97 | - |
oxaloacetate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
17.3 | - |
NH4+ | pH 8.5, 82°C | Archaeoglobus fulgidus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Archaeoglobus fulgidus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Archaeoglobus fulgidus |
76000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
203 | - |
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketocaproate + NH3 + NADH | - |
Archaeoglobus fulgidus | 2-aminocaproate + H2O + NAD+ | - |
? | |
3-fluoropyruvate + NH3 + NADH | - |
Archaeoglobus fulgidus | ? + H2O + NAD+ | - |
? | |
ketovalerate + NH3 + NADH | - |
Archaeoglobus fulgidus | 2-aminovalerate + H2O + NAD+ | - |
? | |
L-2-aminobutyrate + H2O + NAD+ | - |
Archaeoglobus fulgidus | 2-oxobutyrate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Archaeoglobus fulgidus | pyruvate + NH3 + NADH | - |
r | |
L-Asp + H2O + NAD+ | - |
Archaeoglobus fulgidus | oxaloacetate + NH3 + NADH | - |
r | |
L-Ile + H2O + NAD+ | - |
Archaeoglobus fulgidus | 2-oxo-3-methylpentanoate + NH3 + NADH | - |
? | |
L-Ser + H2O + NAD+ | - |
Archaeoglobus fulgidus | 3-hydroxypyruvate + NH3 + NADH | - |
r | |
L-Thr + H2O + NAD+ | - |
Archaeoglobus fulgidus | 3-hydroxy-2-oxobutyrate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | - |
Archaeoglobus fulgidus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
phenylpyruvate + NH3 + NADH | - |
Archaeoglobus fulgidus | L-phenylalanine + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
ALADH | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
82 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
in presence of 1.5 M KCl stable for 3 months | Archaeoglobus fulgidus |
60 | - |
less than 5% loss of activity after 65 h | Archaeoglobus fulgidus |
90 | - |
half-life: 55 h in presence of 1.5 M Kcl | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.1 | - |
NAD+ | pH 8.5, 82°C | Archaeoglobus fulgidus | |
6.1 | - |
L-Ala | pH 8.5, 82°C | Archaeoglobus fulgidus | |
9.6 | - |
L-2-aminobutyrate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
113 | - |
oxaloacetate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
118 | - |
NADH | pH 8.5, 82°C | Archaeoglobus fulgidus | |
118 | - |
pyruvate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
118 | - |
NH4+ | pH 8.5, 82°C | Archaeoglobus fulgidus | |
143 | - |
2-oxobutyrate | pH 8.5, 82°C | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.4 | oxidative deamination | Archaeoglobus fulgidus |
7.5 | - |
reductive amination | Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 8.3 | pH 6.1: about 60% of maximal activity, pH 8.3: about 60% of maximal activity, reductive amination | Archaeoglobus fulgidus |
6.2 | 8 | pH 6.2: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, oxidative deamination | Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADP+ does not serve as coenzyme | Archaeoglobus fulgidus | |
NADH | NADPH does not serve as coenzyme | Archaeoglobus fulgidus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NADH | pH 8.5, 82°C | Archaeoglobus fulgidus | |
4.7 | - |
L-2-aminobutyrate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
4.8 | - |
2-Ketobutyrate | pH 8.5, 82°C | Archaeoglobus fulgidus | |
16.8 | - |
L-Ala | pH 8.5, 82°C | Archaeoglobus fulgidus | |
27.8 | - |
pyruvate | pH 8.5, 82°C | Archaeoglobus fulgidus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Archaeoglobus fulgidus | calculation from nucleotide sequence | - |
4.9 |