Cloned (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Crystallization (Comment) | Organism |
---|---|
in complex with the reaction product 1,4-androstadiene-3,17-dione, to 2.3 A resolution. Key active site residues are Tyr119, Tyr318, Tyr487, and Gly491 | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
Y119F | almost complete loss of activity | Rhodococcus erythropolis |
Y318F | almost complete loss of activity | Rhodococcus erythropolis |
Y487F | almost complete loss of activity | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
4-androstene-3,17-dione | wild-type, pH 8.0, 22°C | Rhodococcus erythropolis | |
0.193 | - |
4-androstene-3,17-dione | mutant Y487F, pH 8.0, 22°C | Rhodococcus erythropolis | |
0.335 | - |
4-androstene-3,17-dione | mutant Y119F, pH 8.0, 22°C | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q9RA02 | - |
- |
Rhodococcus erythropolis SQ1 | Q9RA02 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72.9 | - |
pH 8.0, 22°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis SQ1 | 9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol | - |
? | |
additional information | in catalytic mechanism Tyr487 and Gly491 work in tandem to promote keto-enol tautomerization and increase the acidity of the C2 hydrogen atoms of the substrate. With assistance of Tyr119, the general base Tyr318 abstracts the axialbeta-hydrogen from C2 as a proton, whereas the FAD accepts the axial alpha-hydrogen from the C1 atom of the substrate as a hydride ion | Rhodococcus erythropolis | ? | - |
? | |
additional information | in catalytic mechanism Tyr487 and Gly491 work in tandem to promote keto-enol tautomerization and increase the acidity of the C2 hydrogen atoms of the substrate. With assistance of Tyr119, the general base Tyr318 abstracts the axialbeta-hydrogen from C2 as a proton, whereas the FAD accepts the axial alpha-hydrogen from the C1 atom of the substrate as a hydride ion | Rhodococcus erythropolis SQ1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA1-KSTD1 | - |
Rhodococcus erythropolis |
KSTD1 | - |
Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
4-androstene-3,17-dione | mutant Y119F, pH 8.0, 22°C | Rhodococcus erythropolis | |
1.7 | - |
4-androstene-3,17-dione | mutant Y487F, pH 8.0, 22°C | Rhodococcus erythropolis | |
68.1 | - |
4-androstene-3,17-dione | wild-type, pH 8.0, 22°C | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the C1 and C2 atoms of the substrate are at reaction distance to the N5 atom of the isoalloxazine ring of FAD and the hydroxyl group of Tyr318, respectively, whereas the C3 carbonyl group is at hydrogen bonding distance from the hydroxyl group of Tyr487 and the backbone amide of Gly491 | Rhodococcus erythropolis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
4-androstene-3,17-dione | mutant Y119F, pH 8.0, 22°C | Rhodococcus erythropolis | |
9.1 | - |
4-androstene-3,17-dione | mutant Y487F, pH 8.0, 22°C | Rhodococcus erythropolis | |
1838 | - |
4-androstene-3,17-dione | wild-type, pH 8.0, 22°C | Rhodococcus erythropolis |