Literature summary for 1.3.5.2 extracted from

Rodriguez, J.M.O.; Krupinska, E.; Wacklin-Knecht, H.; Knecht, W.
Preparation of human dihydroorotate dehydrogenase for interaction studies with lipid bilayers (2020), Nucleosides Nucleotides Nucleic Acids, 39, 1306-1319 .

Application

Application	Comment	Organism
synthesis	expression of full-length and N-terminally trucated enzyme lacking 29 amino acids in an Escherichia coli system	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	cardiolipin enhances the interaction of truncated DHODH with lipid bilayers, but the presence of the transmembrane domain in human DHODH is necessary for stable binding to and securing its location at the outer surface of the inner mitochondrial membrane	Homo sapiens	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q02127	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein using immobilized Ni2+ affinity chromatography, full-length protein is solubilized by dodecyl D-maltoside	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
solubilization of full-length enzyme using by dodecyl D-maltoside in order to obtain planar lipid bilayers that are detergent-free. The presence of tetraoleyl cardiolipin in the bilayers significantly enhances the binding between the bilayer and truncated human DHODH. Addition of coenzyme Q10 does not have a detectable effect when compared to bilayers consisting only of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine	Homo sapiens

Renatured (Comment)

Organism

solubilization of full-length enzyme using by dodecyl D-maltoside in order to obtain planar lipid bilayers that are detergent-free. The presence of tetraoleyl cardiolipin in the bilayers significantly enhances the binding between the bilayer and truncated human DHODH. Addition of coenzyme Q10 does not have a detectable effect when compared to bilayers consisting only of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine

Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
69	-	recombinant full-length enzyme, pH 8.0, 30°C	Homo sapiens
94	-	recombinant N-terminally truncated enzyme, pH 8, 30°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-dihydroorotate + decylubiquinone	-	Homo sapiens	orotate + reduced decylubiquinone	-	?