Cloned (Comment) | Organism |
---|---|
the coding regions of LPOR (amino acids 35-397) is amplified from pBDCrPCYA1-FDBR vector, cloned into pGADT7 vector, and introduced into Y187 yeast cells, recombinant expression of MBP-tagged enzyme LPOR. Yeast two-hybrid and pull down assay analyses of protein-protein interaction | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + NADP+ | Chlamydomonas reinhardtii | - |
protochlorophyllide + NADPH + H+ | - |
r | |
chlorophyllide a + NADP+ | Chlamydomonas reinhardtii 4A+ | - |
protochlorophyllide + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | Q39617 | - |
- |
Chlamydomonas reinhardtii 4A+ | Q39617 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + NADP+ | - |
Chlamydomonas reinhardtii | protochlorophyllide + NADPH + H+ | - |
r | |
chlorophyllide a + NADP+ | - |
Chlamydomonas reinhardtii 4A+ | protochlorophyllide + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Light-dependent protochlorophyllide oxidoreductase | - |
Chlamydomonas reinhardtii |
LPOR | - |
Chlamydomonas reinhardtii |
protochlorophyllide reductase | - |
Chlamydomonas reinhardtii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Chlamydomonas reinhardtii | |
NADPH | - |
Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
metabolism | ferredoxin-dependent biliverdin reductase, PCYA1 (EC 1.3.7.5), is a key enzyme involved in the biosynthesis of bilins, mechanism of bilin-mediated regulation of chlorophyll biosynthesis, and regulatory mechanisms of tetrapyrrole biosynthesis in Chlamydomonas reinhardtii, overview. Chlamydomonas PCYA1 uniquely interacts with light-dependent protochlorophyllide oxidoreductase LPOR (protochlorophyllide reductase, EC 1.3.1.33) via its FDBR domain, but not with ferredoxin:protochlorophyllide reductase DPOR (EC 1.3.7.7). This interaction is specific to Chlamydomonas since the Arabidopsis thaliana homologous proteins do not interact with each other, yeast two-hybrid and pull down assay analyses of protein-protein interaction | Chlamydomonas reinhardtii |
physiological function | light-dependent protochlorophyllide oxidoreductase (LPOR) is a rate-limiting key chlorophyll biosynthetic enzyme | Chlamydomonas reinhardtii |