Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Oryza sativa | |
D-fructose 6-phosphate | - |
Oryza sativa | |
D-glucose 6-phosphate | - |
Oryza sativa |
General Stability | Organism |
---|---|
isoforms OxO1-4 are resistant to a treatment with pepsin at 37°C for 30 min at pH 2.0, while the activity of isoform OxO4 is actually increased on pepsin treatment | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | - |
Oryza sativa | |
FeSO4 | - |
Oryza sativa | |
KNO3 | - |
Oryza sativa | |
additional information | not influenced by riboflavin and FMN | Oryza sativa | |
NaNO3 | - |
Oryza sativa | |
NH4NO3 | - |
Oryza sativa | |
oxaloacetic acid | - |
Oryza sativa | |
pyruvate | - |
Oryza sativa | |
SDS | isoforms OxO1-4, are very sensitive to 0.1% SDS with a nearly total loss of their enzyme activities | Oryza sativa | |
ZnSO4 | - |
Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
oxalate | isoform OXO1, at pH 4.0 and 25°C | Oryza sativa | |
0.185 | - |
oxalate | isoform OXO2, at pH 3.5 and 25°C | Oryza sativa | |
0.225 | - |
oxalate | isoform OXO4, at pH 4.0 and 25°C | Oryza sativa | |
0.303 | - |
oxalate | isoform OXO3, at pH 3.0 and 25°C | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na2SO4, EDTA-Na2, KCl, NaCl, K2CO3, Na2CO3, Na2HPO4 and NaH2PO4 have no significant effect on the enzyme activities | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
- |
Oryza sativa |
24100 | - |
- |
Oryza sativa |
24200 | - |
- |
Oryza sativa |
24300 | - |
- |
Oryza sativa |
25900 | - |
- |
Oryza sativa |
27100 | - |
- |
Oryza sativa |
27200 | - |
- |
Oryza sativa |
27400 | - |
- |
Oryza sativa |
222700 | - |
isoform OXO3, gel filtration | Oryza sativa |
235100 | - |
isoform OXO1, gel filtration | Oryza sativa |
287000 | - |
isoform OXO2, gel filtration | Oryza sativa |
295700 | - |
isoform OXO4, gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxalate + O2 + H+ | Oryza sativa | - |
CO2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-25 gel filtration | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no detectable activity against other related organic acids including citrate, glyoxylate, malonate, succinate, glutarate, malate, glycolate, acetate, lactate and formate | Oryza sativa | ? | - |
? | |
oxalate + O2 + H+ | - |
Oryza sativa | CO2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodecamer | 10 * 24100, isoform OXO1, calculated from amino acid sequence | Oryza sativa |
homodecamer | 10 * 24200, isoform OXO4, calculated from amino acid sequence | Oryza sativa |
homodecamer | 10 * 24300, isoform OXO2, calculated from amino acid sequence | Oryza sativa |
homodecamer | 10 * 25900, isoform OXO1, SDS-PAGE | Oryza sativa |
homodecamer | 10 * 27200, isoform OXO2, SDS-PAGE | Oryza sativa |
homodecamer | 10 * 27400, isoform OXO4, SDS-PAGE | Oryza sativa |
homooctamer | 8 * 24000, isoform OXO3, calculated from amino acid sequence | Oryza sativa |
homooctamer | 8 * 27100, isoform OXO3, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
OXO1 | isoform | Oryza sativa |
OXO2 | isoform | Oryza sativa |
OXO3 | isoform | Oryza sativa |
OXO4 | isoform | Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
isoforms OxO2 and OxO3 still maintain all their activities after being heated at 70°C for 1 h while isoforms OxO1 and OxO4 lose about 30% of their activities | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
isoform OXO3, in succinic acid-NaOH buffer | Oryza sativa |
3.5 | - |
isoform OXO2, in succinic acid-NaOH buffer | Oryza sativa |
4 | - |
isoforms OXO1 and OXO4, | Oryza sativa |
8.5 | - |
isoforms OXO1 and OXO4, in Tris-HCl buffer | Oryza sativa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1023 | - |
isoform OXO1, at pH 4.0 and 25°C | Oryza sativa | oxalate | |
1504 | - |
isoform OXO4, at pH 4.0 and 25°C | Oryza sativa | oxalate | |
1892 | - |
isoform OXO3, at pH 3.0 and 25°C | Oryza sativa | oxalate | |
2446 | - |
isoform OXO2, at pH 3.5 and 25°C | Oryza sativa | oxalate |