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Literature summary for 1.2.3.4 extracted from

  • Li, X.C.; Liao, Y.Y.; Leung, D.W.; Wang, H.Y.; Chen, B.L.; Peng, X.X.; Liu, E.E.
    Divergent biochemical and enzymatic properties of oxalate oxidase isoforms encoded by four similar genes in rice (2015), Phytochemistry, 118, 216-223.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate
-
Oryza sativa
D-fructose 6-phosphate
-
Oryza sativa
D-glucose 6-phosphate
-
Oryza sativa

General Stability

General Stability Organism
isoforms OxO1-4 are resistant to a treatment with pepsin at 37°C for 30 min at pH 2.0, while the activity of isoform OxO4 is actually increased on pepsin treatment Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Oryza sativa
FeSO4
-
Oryza sativa
KNO3
-
Oryza sativa
additional information not influenced by riboflavin and FMN Oryza sativa
NaNO3
-
Oryza sativa
NH4NO3
-
Oryza sativa
oxaloacetic acid
-
Oryza sativa
pyruvate
-
Oryza sativa
SDS isoforms OxO1-4, are very sensitive to 0.1% SDS with a nearly total loss of their enzyme activities Oryza sativa
ZnSO4
-
Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
oxalate isoform OXO1, at pH 4.0 and 25°C Oryza sativa
0.185
-
oxalate isoform OXO2, at pH 3.5 and 25°C Oryza sativa
0.225
-
oxalate isoform OXO4, at pH 4.0 and 25°C Oryza sativa
0.303
-
oxalate isoform OXO3, at pH 3.0 and 25°C Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Na2SO4, EDTA-Na2, KCl, NaCl, K2CO3, Na2CO3, Na2HPO4 and NaH2PO4 have no significant effect on the enzyme activities Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
-
Oryza sativa
24100
-
-
Oryza sativa
24200
-
-
Oryza sativa
24300
-
-
Oryza sativa
25900
-
-
Oryza sativa
27100
-
-
Oryza sativa
27200
-
-
Oryza sativa
27400
-
-
Oryza sativa
222700
-
isoform OXO3, gel filtration Oryza sativa
235100
-
isoform OXO1, gel filtration Oryza sativa
287000
-
isoform OXO2, gel filtration Oryza sativa
295700
-
isoform OXO4, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + O2 + H+ Oryza sativa
-
CO2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 gel filtration Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no detectable activity against other related organic acids including citrate, glyoxylate, malonate, succinate, glutarate, malate, glycolate, acetate, lactate and formate Oryza sativa ?
-
?
oxalate + O2 + H+
-
Oryza sativa CO2 + H2O2
-
?

Subunits

Subunits Comment Organism
homodecamer 10 * 24100, isoform OXO1, calculated from amino acid sequence Oryza sativa
homodecamer 10 * 24200, isoform OXO4, calculated from amino acid sequence Oryza sativa
homodecamer 10 * 24300, isoform OXO2, calculated from amino acid sequence Oryza sativa
homodecamer 10 * 25900, isoform OXO1, SDS-PAGE Oryza sativa
homodecamer 10 * 27200, isoform OXO2, SDS-PAGE Oryza sativa
homodecamer 10 * 27400, isoform OXO4, SDS-PAGE Oryza sativa
homooctamer 8 * 24000, isoform OXO3, calculated from amino acid sequence Oryza sativa
homooctamer 8 * 27100, isoform OXO3, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
OXO1 isoform Oryza sativa
OXO2 isoform Oryza sativa
OXO3 isoform Oryza sativa
OXO4 isoform Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
isoforms OxO2 and OxO3 still maintain all their activities after being heated at 70°C for 1 h while isoforms OxO1 and OxO4 lose about 30% of their activities Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
isoform OXO3, in succinic acid-NaOH buffer Oryza sativa
3.5
-
isoform OXO2, in succinic acid-NaOH buffer Oryza sativa
4
-
isoforms OXO1 and OXO4, Oryza sativa
8.5
-
isoforms OXO1 and OXO4, in Tris-HCl buffer Oryza sativa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1023
-
isoform OXO1, at pH 4.0 and 25°C Oryza sativa oxalate
1504
-
isoform OXO4, at pH 4.0 and 25°C Oryza sativa oxalate
1892
-
isoform OXO3, at pH 3.0 and 25°C Oryza sativa oxalate
2446
-
isoform OXO2, at pH 3.5 and 25°C Oryza sativa oxalate