Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Formamide | specific activity of the wild type oxalate oxidase is slightly higher when 1 M formamide is added | Hordeum vulgare |
Cloned (Comment) | Organism |
---|---|
the mutant proteins expressed in Picha pastoris | Hordeum vulgare |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method at 18 °C using 1 micro l of protein (10-15 mg/ml) plus 1 micro l reservoir drops an 1-ml reservoirs, native crystals grown from 2.3M (NH4)2SO4 and 5% 2-propanol are rhombohedral, crystals of recombinant protein, grown from 10% polyethylene glycol 4000 and 0.1 M NaAc, pH 4.6, are tetragonal, Asn75-> Ala OXO are grown from 20% 2-propanol, 0.1 M NaAc, pH 4.6, 0.2 M CaCl2, can be cryo-cooled directly and are rhombohedral | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
N75A | reduced activity to 2.4% of the wild type level | Hordeum vulgare |
N85A | reduced activity to 0.5% of the wild type level | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | for wild type oxalate oxidase, glycolate does not serve as a substrate and does not significantly inhibit turnover when included in the assay at equimolar (20mM) concentrations of oxalate | Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NH4Cl | Hordeum vulgare | |
NH4Cl | specific activity of the wild type oxalate oxidase is lower in the presence of 1 M NaCl | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | P45850 | barley | - |
Purification (Comment) | Organism |
---|---|
mutant proteins | Hordeum vulgare |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.055 | - |
asparagine mutant (N85A), thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is higher in the presence of 1 M NH4Cl or 1 M NaCl and slightly lower when 1 M formamide is added | Hordeum vulgare |
0.24 | - |
asparagine mutant (N75A),thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4, specific activity is slightly higher in the presence of 1 M NH4Cl or 1 M NaCl and does not change when 1 M formamide is added | Hordeum vulgare |
10.26 | - |
wild type, thermostated (25°C) Clark oxygen electrode calibrated with the protocatechuic acid/protocatechuate dioxygenase reaction, pH 4 | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glycolate does not serve as a substrate | Hordeum vulgare | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OXO | - |
Hordeum vulgare |