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Literature summary for 1.2.3.4 extracted from
- Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular, catalytic, and binding properties (1997), Arch. Biochem. Biophys., 340, 239-249.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca(CH3COO)2 | mM concentration 71% activation | Hordeum vulgare |  |
| Cu(CH3COO)2 | 0.1 mM concentration 20% activation | Hordeum vulgare | |
| Pb(CH3COO)2 | 1 mM concentration 157% activation | Hordeum vulgare | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | colorimetric or UV-determination of oxalic acid in biological fluids and beverages | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 100% inhibition at 0.01 mM concentration | Hordeum vulgare | |
| Fe(NO3)2 | 1 mM concentration 98% inhibition; Fe(NO3)2 + EDTA 1 mM concentration 96% inhibition | Hordeum vulgare | |
| ferrous acetate | 94% inhibition at 0.01 mM concentration; ferrous acetate + EDTA 96% inhibition at 0.01 mM concentration | Hordeum vulgare | |
| KCl | 14% inhibition at 1 mM concentration | Hordeum vulgare | |
| NaCl | 14% inhibition at mM concentration | Hordeum vulgare | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
oxalic acid | - |
Hordeum vulgare | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
alpha5, 5 * 25000, SDS-PAGE | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34 | - |
H2O2 production | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalic acid + O2 + 2 H+ | - |
Hordeum vulgare | 2 CO2 + H2O2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | alpha5, 5 * 25000, SDS-PAGE | Hordeum vulgare |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, 96% recovery | Hordeum vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Hordeum vulgare |
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