Crystallization (Comment) | Organism |
---|---|
- |
Methanothermus fervidus |
General Stability | Organism |
---|---|
NADP+ stabilizes against thermal inactivation, NAD+ has no effect | Methanothermus fervidus |
salts stabilize against thermal inactivation in the order: potassium phosphate > sodium phosphate > K2SO4 > sodium citrate > KCl > NaCl | Methanothermus fervidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive to pentalenolactone | Methanothermus fervidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADPH | - |
Methanothermus fervidus | |
0.003 | - |
NADP+ | - |
Methanothermus fervidus | |
0.066 | - |
NADPH | - |
Methanothermus fervidus | |
0.65 | - |
NAD+ | - |
Methanothermus fervidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | dependent on | Methanothermus fervidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Methanothermus fervidus |
200000 | - |
gel filtration | Methanothermus fervidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermus fervidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermus fervidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
49.59 | - |
- |
Methanothermus fervidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Methanothermus fervidus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
r | |
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+ | r | Methanothermus fervidus | 1,3-diphosphoglycerate + NAD(P)H | - |
? | |
D-glyceraldehyde-3-phosphate + phosphate + NADP+ | r | Methanothermus fervidus | 1,3-diphosphoglycerate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Methanothermus fervidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
pH 7.0, 30 min, stable | Methanothermus fervidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Methanothermus fervidus |