Crystallization (Comment) | Organism |
---|---|
analysis of the enzyme in complex with a synthetic peptide representing the first FNR-binding repeat from Pisum sativum Tic62, including the strictly conserved core motif KTEQPLSPYTAYDDLKPPSSPSPTKPS, based on the crystal structure PDB ID 1QG0, X-ray diffraction structure analysis at 1.7 A resolution, molecular replacement | Pisum sativum |
General Stability | Organism |
---|---|
interaction with Tic62 stabilizes FNR | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | FNR is a soluble protein associated with thylakoid membranes | Pisum sativum | 9507 | - |
thylakoid membrane | FNR is a soluble protein associated with thylakoid membranes | Pisum sativum | 42651 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + NADP+ | Pisum sativum | - |
2 oxidized ferredoxin + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 reduced ferredoxin + NADP+ | - |
Pisum sativum | 2 oxidized ferredoxin + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | FNR-membrane recruitment motif, FNR-MRM, forms a polyproline helix that interacts with a proline recognition domain on FNR. FNR displays a pH-dependent high affinity to the MRM, loop residues contribute to multiple interactions with both FNR subunits | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin:NADPH oxidoreductase | - |
Pisum sativum |
FNR | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | assay at | Pisum sativum |
General Information | Comment | Organism |
---|---|---|
additional information | FNR interacts with Tic62, a member of the Tic complex, i.e. translocon at the inner envelope of chloroplasts, involved in redox-regulation of protein import into chloroplasts. Tic62 represents a major FNR interaction protein partner at the thylakoids, and binding to Tic62 clearly increases the stability of FNR. The specific interaction with FNR is mediated by a conserved sequence motif rich in proline and serine residues, located in the C terminus of Tic62 | Pisum sativum |