Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
oxidized ferredoxin | Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor | Plasmodium falciparum | |
0.0055 | - |
NADPH | using cytochrome c as electron acceptor | Plasmodium falciparum | |
0.01 | - |
NADPH | using K3Fe(CN)6 as electron acceptor | Plasmodium falciparum | |
0.05 | - |
NADPH | using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | C6KT68 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride | - |
Plasmodium falciparum | NADP+ + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride | - |
? | |
NADPH + K3Fe(CN)6 | - |
Plasmodium falciparum | NADP+ + reduced K3Fe(CN)6 | - |
r | |
NADPH + oxidized cytochrome c | - |
Plasmodium falciparum | NADP+ + reduced cytochrome c | - |
r | |
NADPH + oxidized ferredoxin | - |
Plasmodium falciparum | NADP+ + reduced ferredoxin | - |
? | |
oxidized ferredoxin + NADPH + H+ | - |
Plasmodium falciparum | reduced ferredoxin + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | Under oxidizing conditions, Plasmodium falciparum FNR constitutes an inactive dimeric form stabilized by an intermolecular disulfide bond. Monomer-dimer interconversion can be controlled by oxidizing and reducing agents that are possibly present within the apicoplast, such as H2O2, glutathione, and lipoate. | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NADP+ reductase | - |
Plasmodium falciparum |
FNR | - |
Plasmodium falciparum |
PfFNR | ferredoxin-NADP+ reductase of Plasmodium falciparum | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | - |
NADPH | using cytochrome c as electron acceptor | Plasmodium falciparum | |
65 | - |
NADPH | using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor | Plasmodium falciparum | |
85 | - |
NADPH | using K3Fe(CN)6 as electron acceptor | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
NADPH | using cytochrome c as electron acceptor | Plasmodium falciparum | |
1300 | - |
NADPH | using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor | Plasmodium falciparum | |
8000 | - |
Ferredoxin | Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor | Plasmodium falciparum | |
8500 | - |
NADPH | using K3Fe(CN)6 as electron acceptor | Plasmodium falciparum |