Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 26.6% inhibition at 1 mM | Kluyveromyces marxianus | |
EDTA | 66% inhibition at 1 mM | Kluyveromyces marxianus | |
iodoacetic acid | 23.5% inhibition at 1 mM | Kluyveromyces marxianus | |
KCN | 30% inhibition at 2 mM | Kluyveromyces marxianus | |
additional information | no inhibition of Cu/ZnSOD by 10 mM H2O2, 1 mM CaCl2, and 1 mM NaN3 | Kluyveromyces marxianus | |
NaCl | slight inhibition at 1 mM | Kluyveromyces marxianus | |
SDS | - |
Kluyveromyces marxianus | |
ZnCl2 | slight inhibition at 1 mM | Kluyveromyces marxianus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | a Cu/Zn-SOD | Kluyveromyces marxianus | |
Zn | a Cu/Zn-SOD | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34034 | - |
2 * 34034, sequence calculation and mass spectrometry | Kluyveromyces marxianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | Kluyveromyces marxianus | - |
O2 + H2O2 | - |
? | |
2 O2.- + 2 H+ | Kluyveromyces marxianus NBIMCC 1984 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
- |
- |
Kluyveromyces marxianus NBIMCC 1984 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains carbohydrate at 1200 kDa, it is associated with carbohydrate components comprising at least 4 monosugars, D-xylose, D-mannose, D-galactose and N-acetyl-D-glucosamine | Kluyveromyces marxianus |
Purification (Comment) | Organism |
---|---|
native enzyme 28.5fold from strain NBIMCC 1984 by thermal treatment, dialysis, ion exchange chromatography, and chromatofocusing | Kluyveromyces marxianus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
996.2 | - |
purified native enzyme | Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | - |
Kluyveromyces marxianus | O2 + H2O2 | - |
? | |
2 O2.- + 2 H+ | - |
Kluyveromyces marxianus NBIMCC 1984 | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34034, sequence calculation and mass spectrometry | Kluyveromyces marxianus |
Synonyms | Comment | Organism |
---|---|---|
Cu/Zn SOD | - |
Kluyveromyces marxianus |
Cu/Zn superoxide dismutase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Kluyveromyces marxianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 90 | temperature stability profile, overview | Kluyveromyces marxianus |
60 | 70 | the purified enzyme is quite stable | Kluyveromyces marxianus |
75 | - |
purified enzyme, half-life is 22 min | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Kluyveromyces marxianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | rapid inactivation below pH 6.5 and above pH 8.0, 60% remaiining activity at pH 7.8 after 10 min at 25°C | Kluyveromyces marxianus |