Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determnination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodothermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibition at 10 mM, the Fe-SOD is less sensitive | Rhodothermus sp. | |
KCN | inhibition at 10 mM, the Fe-SOD is less sensitive | Rhodothermus sp. | |
NaN3 | inhibition at 5 mM, the Fe-SOD is less sensitive | Rhodothermus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a Fe-SOD | Rhodothermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23600 | - |
2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE | Rhodothermus sp. |
25000 | - |
2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE | Rhodothermus sp. |
52000 | - |
recombinant enzyme, gel filtration | Rhodothermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | Rhodothermus sp. | - |
O2 + H2O2 | - |
? | |
O2.- + H+ | Rhodothermus sp. XMH10 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus sp. | - |
- |
- |
Rhodothermus sp. XMH10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodothermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | - |
Rhodothermus sp. | O2 + H2O2 | - |
? | |
O2.- + H+ | - |
Rhodothermus sp. XMH10 | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE | Rhodothermus sp. |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Rhodothermus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | recombinant enzyme | Rhodothermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
12 h, purified recombinant enzyme, 90% remaining activity | Rhodothermus sp. |
80 | - |
purified recombinant enzyme, 40% remaining activity after 30 min, complete inactivation after 4 h | Rhodothermus sp. |
90 | - |
purified recombinant enzyme, 10% remaining activity after 30 min | Rhodothermus sp. |
100 | - |
purified recombinant enzyme, complete inactivation after 30 min | Rhodothermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant enzyme | Rhodothermus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | inactivation at pH 2.0, shrap drop of activity below pH 4.0, high activity at pH 4.0-pH 5.0, about 70% of maximal activity at pH 6.0-12.0 | Rhodothermus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodothermus sp. | sequence calcualtion | - |
5.54 |