Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-Hydroxyquinoline-5-sulfonate | Fe-SOD, slightly stimulating, depending on the assay method | Pseudomonas putida | |
o-phenanthroline | Fe-SOD, slightly stimulating | Pseudomonas putida | |
o-phenanthroline | depending on assay method | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
Fe-SOD, dialysis against 55% saturated ammonium sulfate solution, pH 4.5, 1 week at 2°C under reduced pressure | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no inhibition | Pseudomonas putida | |
H2O2 | Fe-SOD | Pseudomonas putida | |
additional information | no inhibition by N-ethylmaleide | Pseudomonas putida | |
o-phenanthroline | depending on assay method; Fe-SOD | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1.0-1.45 mol per mol of enzyme | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19500 | - |
2 * 19500, Fe-SOD, SDS-PAGE | Pseudomonas putida |
40000 | - |
Fe-SOD, gel filtration, sedimentation analysis | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
Fe-SOD | - |
Pseudomonas putida Fe-SOD | - |
Fe-SOD | - |
Purification (Comment) | Organism |
---|---|
Fe-SOD | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 superoxide + 2 H+ = O2 + H2O2 | amino acid composition, comparison | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Pseudomonas putida | O2 + H2O2 | - |
? | |
O2- + H+ | - |
Pseudomonas putida Fe-SOD | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 19500, Fe-SOD, SDS-PAGE | Pseudomonas putida |