Crystallization (Comment) | Organism |
---|---|
enzyme 10 mg per ml in Tris/HCl 50 mM, pH 8.2 by dialysis against ammonium sulfate 2.8 M, pH 8.2, 4°C | Homo sapiens |
General Stability | Organism |
---|---|
SDS, 1%, complete loss of activity after 6 h | Homo sapiens |
urea: 8 M, stable | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | Mn-SOD | Homo sapiens | |
iodoacetamide | Mn-SOD | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21300 | - |
4 * 21300, SDS-PAGE | Homo sapiens |
88000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
Mn-SOD | - |
Homo sapiens Mn-SOD | - |
Mn-SOD | - |
Purification (Comment) | Organism |
---|---|
Mn-SOD from liver | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-35°C, protein concentration 45 mg/ml, 50% glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Homo sapiens | O2 + H2O2 | - |
? | |
O2- + H+ | - |
Homo sapiens Mn-SOD | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 21300, SDS-PAGE | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
5°C, 1 day, 35% loss of activity | Homo sapiens |
7.2 | - |
4°C, 1 day, 2% loss of activity | Homo sapiens |
8 | 9.3 | 4°C, 1 day, 10-20% loss of activity | Homo sapiens |