Cloned (Comment) | Organism |
---|---|
expression using a Brevibacillus-based expression system | Streptomyces griseus subsp. griseus |
Protein Variants | Comment | Organism |
---|---|---|
Y56W | mutation shows no detectable effect on substrate-binding preferences but, in synergy experiments with chitinases, the mutant appears to be more efficient on alpha-chitin | Streptomyces griseus subsp. griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus subsp. griseus | B1VN59 | - |
- |
Streptomyces griseus subsp. griseus JCM 4626 | B1VN59 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-chitin + ascorbate + O2 | enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin | Streptomyces griseus subsp. griseus | C1-oxidized chitooligosaccharides + dehydroascorbate + H2O | products show oxidation of the C1 carbon leading to aldonic acids | ? | |
alpha-chitin + ascorbate + O2 | enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin | Streptomyces griseus subsp. griseus JCM 4626 | C1-oxidized chitooligosaccharides + dehydroascorbate + H2O | products show oxidation of the C1 carbon leading to aldonic acids | ? | |
beta-chitin + ascorbate + O2 | enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin | Streptomyces griseus subsp. griseus | C1-oxidized chitooligosaccharides + dehydroascorbate + H2O | products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids | ? | |
beta-chitin + ascorbate + O2 | enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin | Streptomyces griseus subsp. griseus JCM 4626 | C1-oxidized chitooligosaccharides + dehydroascorbate + H2O | products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids | ? |
Synonyms | Comment | Organism |
---|---|---|
LPMO10F | - |
Streptomyces griseus subsp. griseus |
SGR_6855 | - |
Streptomyces griseus subsp. griseus |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme increases chitin solubilization yields of chitinases by up to 30fold and 20fold for alpha- and beta-chitin, respectively. The addition of LPMO10F leads to a substantial increase in the (GlcNAc)2:GlcNAc product ratio of chitinases, in reactions with alpha-chitin only | Streptomyces griseus subsp. griseus |