KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000367 | - |
ecdysone | microsomal enzyme | Manduca sexta | |
0.0000163 | - |
ecdysone | mitochondrial enzyme | Manduca sexta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Manduca sexta | - |
- |
mitochondrion | - |
Manduca sexta | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Manduca sexta | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | Manduca sexta | last step in biosynthesis of insect molting hormone | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | Manduca sexta | midgut microsomes show higher specific activity with 2fold higher Vmax, while total activity is 8-10fold higher in mitochondria | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
tobacco hornworm | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | microsomal enzyme system contains NADPH-dependent cytochrome P-450 reductase without a second electron carrier type | Manduca sexta | |
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | a cytochrome P-450-dependent steroid hydroxylase as part of the mitochondrial enzyme system with both ferredoxin and cytochrome P-450 components | Manduca sexta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | enzyme is located in mitochondrion | Manduca sexta | - |
integument | - |
Manduca sexta | - |
malpighian tubule | - |
Manduca sexta | - |
midgut | larval, enzyme is located in mitochondrion and microsome | Manduca sexta | - |
salivary gland | - |
Manduca sexta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | - |
Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | last step in biosynthesis of insect molting hormone | Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | midgut microsomes show higher specific activity with 2fold higher Vmax, while total activity is 8-10fold higher in mitochondria | Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Manduca sexta | |
Ferredoxin | isozyme in mitochondria | Manduca sexta | |
NADPH | - |
Manduca sexta |