Cloned (Comment) | Organism |
---|---|
gene dszA, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha | Mycolicibacterium phlei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Mycolicibacterium phlei | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Mycolicibacterium phlei GTIS10 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
additional information | Mycolicibacterium phlei | The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate | ? | - |
? | |
additional information | Mycolicibacterium phlei GTIS10 | The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Mycolicibacterium phlei GTIS10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography | Mycolicibacterium phlei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | the organism can use dibenzothiophene as a sole source of sulfur for growth at 30-52°C, it can also utilize benzothiophene and thiophene as sulfur sources for growth. Range of organosulfur substrates utilized as a sole source of sulfur for growth by Mycobacterium phlei GTIS10, best is dibenzothiophene, overview | Mycolicibacterium phlei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Mycolicibacterium phlei | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Mycolicibacterium phlei GTIS10 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
additional information | The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate | Mycolicibacterium phlei | ? | - |
? | |
additional information | The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate | Mycolicibacterium phlei GTIS10 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Mycolicibacterium phlei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Mycolicibacterium phlei |
General Information | Comment | Organism |
---|---|---|
physiological function | Mycobacterium phlei strain GTIS10 converts dibenzothiophene to 2'-hydroxybiphenyl, determination of metabolites from dibenzothiophene, overview | Mycolicibacterium phlei |