Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be useful in the highly enantioselective in the synthesis of chiral phenyl and benzyl sulfoxides | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Pseudomonas fluorescens | - |
4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | Pseudomonas fluorescens ACB | - |
4-hydroxyphenyl acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
gene hapE | - |
Pseudomonas fluorescens ACB | - |
gene hapE | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | - |
Pseudomonas fluorescens | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
(4-hydroxyphenyl)ethan-1-one + NADPH + O2 | - |
Pseudomonas fluorescens ACB | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
2-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 2-chlorophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
2-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens ACB | 2-chlorophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
2-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 2-chlorophenyl methyl (S)-sulfoxide + NADP+ + H2O | 96% enantiomeric excess | ? | |
2-chloroethyl phenyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 2-chloroethyl phenyl (S)-sulfoxide + NADP+ + H2O | 81% enantiomeric excess | ? | |
3-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 3-chlorophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
3-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens ACB | 3-chlorophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
3-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 3-chlorophenyl methyl (S)-sulfoxide + NADP+ + H2O | 99% enantiomeric excess | ? | |
4-amino-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-aminophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-amino-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens ACB | 4-aminophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-amino-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-[(S)-methylsulfinyl]aniline + NADP+ + H2O | 99% enantiomeric excess | ? | |
4-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-chlorophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-chloro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-chlorophenyl methyl (S)-sulfoxide + NADP+ + H2O | 96% enantiomeric excess | ? | |
4-cyano-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-cyanophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-cyano-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-[(S)-methylsulfinyl]benzonitrile + NADP+ + H2O | 96% enantiomeric excess | ? | |
4-methoxy-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-methoxyphenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-methoxy-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-methoxyphenyl methyl (S)-sulfoxide + NADP+ + H2O | 99% enantiomeric excess | ? | |
4-methyl-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-methylphenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-methyl-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl 4-methylphenyl (S)-sulfoxide + NADP+ + H2O | 99% enantiomeric excess | ? | |
4-nitro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-nitrophenyl methyl sulfoxide + NADP+ + H2O | - |
? | |
4-nitro-thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl 4-nitrophenyl (S)-sulfoxide + NADP+ + H2O | 87% enantiomeric excess | ? | |
benzyl 1-methylethyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | benzyl 1-methylethyl (R)-sulfoxide + NADP+ + H2O | 82% enantiomeric excess | ? | |
benzyl butyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | benzyl butyl (R)-sulfoxide + NADP+ + H2O | 77% enantiomeric excess | ? | |
benzyl ethyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | benzyl ethyl (S)-sulfoxide + NADP+ + H2O | 81% enantiomeric excess | ? | |
benzyl methyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | benzyl methyl (S)-sulfoxide + NADP+ + H2O | 85% enantiomeric excess | ? | |
benzyl propyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | benzyl propyl (R)-sulfoxide + NADP+ + H2O | 65% enantiomeric excess | ? | |
butyl phenyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | butyl phenyl (S)-sulfoxide + NADP+ + H2O | 71% enantiomeric excess | ? | |
chloromethyl phenyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | chloromethyl phenyl (R)-sulfoxide + NADP+ + H2O | 76% enantiomeric excess | ? | |
cyclopropyl phenyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | cyclopropyl phenyl (S)-sulfoxide + NADP+ + H2O | 97% enantiomeric excess | ? | |
ethenyl phenyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | ethenyl phenyl (S)-sulfoxide + NADP+ + H2O | 98% enantiomeric excess | ? | |
ethyl phenyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | ethyl phenyl (S)-sulfoxide + NADP+ + H2O | 99% enantiomeric excess | ? | |
methyl (phenylsulfanyl)methyl ether + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methoxymethyl phenyl (R)-sulfoxide + NADP+ + H2O | 98% enantiomeric excess | ? | |
methyl 2-phenylethyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl 2-phenylethyl (R)-sulfoxide + NADP+ + H2O | 51% enantiomeric excess | ? | |
methyl 3-phenylpropyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl 3-phenylpropyl (R)-sulfoxide + NADP+ + H2O | 57% enantiomeric excess | ? | |
methyl naphthalen-2-yl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl naphthalen-2-yl (S)-sulfoxide + NADP+ + H2O | 95% enantiomeric excess | ? | |
additional information | the recombinant enzyme is highly enantioselective in the synthesis of chiral phenyl and benzyl sulfoxides, oxidation of aromatic sulfides, substrate specificity and enantioselectivity, overview | Pseudomonas fluorescens | ? | - |
? | |
additional information | the recombinant enzyme is highly enantioselective in the synthesis of chiral phenyl and benzyl sulfoxides, oxidation of aromatic sulfides, substrate specificity and enantioselectivity, overview | Pseudomonas fluorescens ACB | ? | - |
? | |
phenyl prop-2-en-1-yl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | phenyl prop-2-en-1-yl (S)-sulfoxide + NADP+ + H2O | 98% enantiomeric excess | ? | |
phenyl propyl sulfide + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | phenyl propyl (S)-sulfoxide + NADP+ + H2O | 97% enantiomeric excess | ? | |
thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl phenyl sulfoxide + NADP+ + H2O | - |
? | |
thioanisole + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | methyl phenyl (S)-sulfoxide + NADP+ + H2O | 99% enantiomeric excess | ? |
Synonyms | Comment | Organism |
---|---|---|
HAPMO | - |
Pseudomonas fluorescens |
More | the enzyme is a BaeyerVilliger monooxygenase | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme | Pseudomonas fluorescens | |
NADPH | dependent on | Pseudomonas fluorescens |