KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
L-arginine | isoform III | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Mus musculus | - |
- |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Homo sapiens | - |
- |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Rattus norvegicus | - |
- |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Sus scrofa | - |
- |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
x * 160000, isoform I, amino acid sequence determination | Homo sapiens |
160000 | - |
x * 160000, isoform I, amino acid sequence determination | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Mus musculus | physiological functions and pathophysiology of the isoforms | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Homo sapiens | physiological functions and pathophysiology of the isoforms | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | physiological functions and pathophysiology of the isoforms | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Sus scrofa | physiological functions and pathophysiology of the isoforms | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Bos taurus | physiological functions and pathophysiology of the isoforms | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | isoform I and II are regulated by Ca2+/calmodulin, isoform II is Ca2+-independent, but requires calmodulin, inducible by cytokines | Mus musculus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | isoform I and II are regulated by Ca2+/calmodulin, isoform II is Ca2+-independent, but requires calmodulin, inducible by cytokines | Homo sapiens | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | isoform I and II are regulated by Ca2+/calmodulin, isoform II is Ca2+-independent, but requires calmodulin, inducible by cytokines | Rattus norvegicus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | isoform I and II are regulated by Ca2+/calmodulin, isoform II is Ca2+-independent, but requires calmodulin, inducible by cytokines | Sus scrofa | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | isoform I and II are regulated by Ca2+/calmodulin, isoform II is Ca2+-independent, but requires calmodulin, inducible by cytokines | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Bos taurus | - |
adrenal gland | isoform I | Homo sapiens | - |
adrenal gland | isoform I | Rattus norvegicus | - |
adrenal gland | isoform I | Sus scrofa | - |
brain | - |
Mus musculus | - |
brain | - |
Bos taurus | - |
brain | isoform I | Homo sapiens | - |
brain | isoform I | Rattus norvegicus | - |
brain | isoform I | Sus scrofa | - |
chondrocyte | - |
Homo sapiens | - |
DLD-1 cell | - |
Homo sapiens | - |
endothelium | isoform III | Mus musculus | - |
endothelium | isoform III | Homo sapiens | - |
endothelium | isoform III | Rattus norvegicus | - |
endothelium | isoform III | Sus scrofa | - |
endothelium | isoform III | Bos taurus | - |
endothelium | liver, lung, adrenal glandcolon, isoform II | Mus musculus | - |
endothelium | liver, lung, adrenal glandcolon, isoform II | Homo sapiens | - |
endothelium | liver, lung, adrenal glandcolon, isoform II | Rattus norvegicus | - |
endothelium | lung, uterus, stomach | Homo sapiens | - |
endothelium | lung, uterus, stomach | Rattus norvegicus | - |
endothelium | lung, uterus, stomach | Sus scrofa | - |
hepatocyte | isoform II | Mus musculus | - |
hepatocyte | isoform II | Homo sapiens | - |
hepatocyte | isoform II | Rattus norvegicus | - |
kidney | macula densa cells, isoform I | Homo sapiens | - |
kidney | macula densa cells, isoform I | Rattus norvegicus | - |
kidney | macula densa cells, isoform I | Sus scrofa | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
macrophage | - |
Bos taurus | - |
macrophage | in liver, lung, kidney, isoform II | Mus musculus | - |
macrophage | in liver, lung, kidney, isoform II | Homo sapiens | - |
macrophage | in liver, lung, kidney, isoform II | Rattus norvegicus | - |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Mus musculus | - |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Homo sapiens | - |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Rattus norvegicus | - |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Sus scrofa | - |
additional information | 3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells | Bos taurus | - |
neuron | - |
Mus musculus | - |
neuron | peripheral nitrergic nerves, isoform I | Homo sapiens | - |
neuron | peripheral nitrergic nerves, isoform I | Rattus norvegicus | - |
neuron | peripheral nitrergic nerves, isoform I | Sus scrofa | - |
pancreas | - |
Bos taurus | - |
pancreas | islet cells, isoform I | Homo sapiens | - |
pancreas | islet cells, isoform I | Rattus norvegicus | - |
pancreas | islet cells, isoform I | Sus scrofa | - |
smooth muscle | - |
Rattus norvegicus | - |
spinal cord | isoform I | Homo sapiens | - |
spinal cord | isoform I | Rattus norvegicus | - |
spinal cord | isoform I | Sus scrofa | - |
spleen | red pulp, eosinophils and neutrophils, isoform II | Mus musculus | - |
spleen | red pulp, eosinophils and neutrophils, isoform II | Homo sapiens | - |
spleen | red pulp, eosinophils and neutrophils, isoform II | Rattus norvegicus | - |
sympathetic ganglion | isoform I | Homo sapiens | - |
sympathetic ganglion | isoform I | Rattus norvegicus | - |
sympathetic ganglion | isoform I | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Sus scrofa | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | physiological functions and pathophysiology of the isoforms | Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | physiological functions and pathophysiology of the isoforms | Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | physiological functions and pathophysiology of the isoforms | Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | physiological functions and pathophysiology of the isoforms | Sus scrofa | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | physiological functions and pathophysiology of the isoforms | Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Sus scrofa |
? | x * 160000, isoform I, amino acid sequence determination | Homo sapiens |
? | x * 160000, isoform I, amino acid sequence determination | Rattus norvegicus |
? | 130000-133000, isoforms II and III, amino acid sequence determination | Mus musculus |
? | 130000-133000, isoforms II and III, amino acid sequence determination | Homo sapiens |
? | 130000-133000, isoforms II and III, amino acid sequence determination | Rattus norvegicus |
? | 130000-133000, isoforms II and III, amino acid sequence determination | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
cb-NOS | isoform I, constitutive, from brain | Homo sapiens |
cb-NOS | isoform I, constitutive, from brain | Rattus norvegicus |
cb-NOS | isoform I, constitutive, from brain | Sus scrofa |
EC-NOS | isoform III, in endothelial cells | Mus musculus |
EC-NOS | isoform III, in endothelial cells | Homo sapiens |
EC-NOS | isoform III, in endothelial cells | Bos taurus |
i-NOS | isoform II, inducible | Mus musculus |
i-NOS | isoform II, inducible | Homo sapiens |
i-NOS | isoform II, inducible | Rattus norvegicus |
n-NOS | isoform I, neuronal enzyme | Homo sapiens |
n-NOS | isoform I, neuronal enzyme | Rattus norvegicus |
n-NOS | isoform I, neuronal enzyme | Sus scrofa |