Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrocatechol + NAD(P)H + H+ + O2 | Lysinibacillus sphaericus | - |
2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? | |
4-nitrocatechol + NAD(P)H + H+ + O2 | Arthrobacter sp. JS443 | - |
2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? | |
4-nitrocatechol + NAD(P)H + H+ + O2 | Lysinibacillus sphaericus JS905 | - |
2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. JS443 | A7YVV2 | - |
- |
Lysinibacillus sphaericus | Q6F4M8 AND Q6F4M9 | genes npcA and npcB | - |
Lysinibacillus sphaericus JS905 | Q6F4M8 AND Q6F4M9 | genes npcA and npcB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrocatechol + NAD(P)H + H+ + O2 | - |
Lysinibacillus sphaericus | 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? | |
4-nitrocatechol + NAD(P)H + H+ + O2 | - |
Arthrobacter sp. JS443 | 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? | |
4-nitrocatechol + NAD(P)H + H+ + O2 | - |
Lysinibacillus sphaericus JS905 | 2-hydroxy-1,4-benzoquinone + nitrite + NAD(P)+ + H2O | - |
? | |
4-nitrocatechol + NADH + H+ + O2 | - |
Lysinibacillus sphaericus | 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O | - |
? | |
4-nitrocatechol + NADH + H+ + O2 | - |
Arthrobacter sp. JS443 | 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O | - |
? | |
4-nitrocatechol + NADH + H+ + O2 | - |
Lysinibacillus sphaericus JS905 | 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase | UniProt | Lysinibacillus sphaericus |
4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase | UniProt | Arthrobacter sp. JS443 |
NpcA | - |
Lysinibacillus sphaericus |
npcB | - |
Lysinibacillus sphaericus |
PNP monooxygenase | - |
Lysinibacillus sphaericus |
PNP monooxygenase | - |
Arthrobacter sp. JS443 |
two-component PNP monooxygenase | - |
Lysinibacillus sphaericus |
two-component PNP monooxygenase | - |
Arthrobacter sp. JS443 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Lysinibacillus sphaericus | |
FAD | - |
Arthrobacter sp. JS443 | |
NADH | - |
Lysinibacillus sphaericus | |
NADH | - |
Arthrobacter sp. JS443 |
General Information | Comment | Organism |
---|---|---|
evolution | PNP monooxygenase belongs to a two-component flavin-diffusible monooxygenase family | Lysinibacillus sphaericus |
metabolism | the enzyme PNP monoxygenase is involved in the degradation of 4-nitrophenol, proposed pathway, overview. 4-Nitrophenol is converted to 4-nitrocatechol by a 4-nitrophenol 2-monooxygenase, EC 1.14.13.29, of the enzyme, which is subsequently converted to 2-hydroxy-1,4-benzoquinone, EC 1.14.13.166 | Lysinibacillus sphaericus |
metabolism | the enzyme PNP monoxygenase is involved in the degradation of 4-nitrophenol, proposed pathway, overview. 4-Nitrophenol is converted to 4-nitrocatechol by a 4-nitrophenol 2-monooxygenase, EC 1.14.13.29, of the enzyme, which is subsequently converted to 2-hydroxy-1,4-benzoquinone, EC 1.14.13.166 | Arthrobacter sp. JS443 |
additional information | enzyme structure homology model for PNP monooxygenase using crystal structure of chlorophenol 4-monooxygenase from Burkholderia cepacia AC1100, PDB IS 3HWC, as template. Molecular dynamics simulations performed for docking complexes show the stable interaction between enzyme and substrate 4-nitrocatechol. Docking of substrates into the active site of PNP monooxygenase, Arg100, Gln158 and Thr193 are the key catalytic residues, overview | Lysinibacillus sphaericus |
additional information | enzyme structure homology model for PNP monooxygenase using crystal structure of chlorophenol 4-monooxygenase from Burkholderia cepacia AC1100, PDB IS 3HWC, as template. Molecular dynamics simulations performed for docking complexes show the stable interaction between enzyme and substrate 4-nitrocatechol. of substrates into the active site of PNP monooxygenase, overview | Arthrobacter sp. JS443 |
physiological function | the enzyme comprises two components, a flavoprotein reductase and an oxygenase, catalyzes the initial two sequential monooxygenations to convert 4-nitrophenol to trihydroxybenzene, EC 1.14.13.29 and EC 1.14.13.166 | Lysinibacillus sphaericus |
physiological function | the enzyme comprises two components, a flavoprotein reductase and an oxygenase, catalyzes the initial two sequential monooxygenations to convert 4-nitrophenol to trihydroxybenzene, EC 1.14.13.29 and EC 1.14.13.166 | Arthrobacter sp. JS443 |