Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hemin | maximal activity at 0.001 mM | Escherichia coli |
General Stability | Organism |
---|---|
stabilizing of activity during gel filtration by adding 10 mM NaN3 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCN | 45% of control activity with 0.025 mM, 2% with 0.25 mM | Escherichia coli | |
Superoxide dismutase | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
strain AB1157 | Escherichia coli |
0.865 | - |
mutant strain PG118 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NO + O2 + NAD(P)H | - |
Escherichia coli | NO3- + NAD(P)+ + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | full activity at 0.001 mM | Escherichia coli | |
NADPH | - |
Escherichia coli | |
O2 | - |
Escherichia coli |