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Literature summary for 1.14.11.55 extracted from

  • Zhao, Q.; Li, S.; Lv, P.; Sun, S.; Ma, C.; Xu, P.; Su, H.; Yang, C.
    High ectoine production by an engineered Halomonas hydrothermalis Y2 in a reduced salinity medium (2019), Microb. Cell Fact., 18, 184 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis in an optimized medium containing 100 g/l NaCl in a 500-ml flask, the double mutant lacking EctD and ectoine hydrolaseDoeA synthesizes 3.13 g/l ectoine after 30 h cultivation. Mutants additionally lacking key Na+/H+ antiporter Mrp can synthesize around 7 g/l or 500 mg/(g DCW) in the medium containing lower concentration of NaCl. During a fed-batch fermentation process with 60 g/l NaCl stress, a maximum 10.5 g/l ectoine is accumulated by the Mrp-deficient strain,with a specific production of 765 mg/(g DCW) and a yield of 0.21 g/g monosodium glutamate Halomonas hydrothermalis

Organism

Organism UniProt Comment Textmining
Halomonas hydrothermalis
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Halomonas hydrothermalis Y2
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